新疆和田羊与策勒黑羊Mel分泌和MTRla表达的时空差异性研究

We researched on the spatiotemporal Differences of Melatonin secretion,MTRla distribution and expression of seasonally breeding-Hetian sheep and perennially breeding-Cele black sheep in Xinjiang,then revealed the effect differences of MTR1a to Hetian sheep and Cele black sheep in propagation neuroendocrine regulation and reproductive behavior.That can illuminated the polyembryonic mechanism in sheep,then provided basic work to perfect the theory of sheep,s estrus and ovulation,which will not only atestablish the theoretical foundation for the new Induction technology of sheep,s estrus and ovulation ,but also the genetic breeding technology to improve sheep,s fertility. Furthermore,it is important for protectting the diversity of local fine varieties. According to study of the response in blood MEL level of sheep to spatiotemporal Differences,and the effect of spatiotemporal Differences on MTR1a distribution and expression level in sheep,s tissues,and the interaction mechanism of MEL level and MTR1a expression level in tissues and organs，and it will reveal the effect of interaction on ovulation rate, embryo survival rate and lambing rate in Hetian sheep and Cele black Sheep.Furthermore, that also supplies a theory basis for the model of perenniall breeding and management of Hetian Sheep.We will publish 4-6 research paper after the completion of the project, among which 1-2 articles are cited by SCI and 3-4 by GCJC. Meanwhile we will bring up three young teachers and one postgraduate students.

研究南疆地区季节性繁殖的和田羊和常年繁殖的策勒黑羊褪黑素释放及褪黑素受体MTR1a分布与表达的时空差异，继而揭示褪黑素受体对和田羊和策勒黑羊繁殖神经内分泌调节及繁殖行为的影响差异，不但可以阐明绵羊多胎机制，为完善羊发情排卵调控理论提供依据，从而为开发诱导羊发情排卵新技术和提高羊繁殖力的技术奠定理论基础；而且在保护地方优良品种的多样性上有着重要的意义。本研究通过研究和田羊和策勒黑羊血液褪黑激素水平对时空差异的响应、时空差异对和田羊和策勒黑羊组织器官中褪黑激素受体分布及表达水平的影响、和田羊和策勒羊褪黑激素与组织器官中MTR1a表达的互作机制，以及二者互作对和田羊和策勒黑羊排卵率、胚胎存活率和产羔率的影响，并由此为建立和田羊全年繁殖的饲养及管理模式提供理论依据。项目完成后，将撰写研究论文4-6篇，其中SCI收录1-2篇，中文核心期刊3-4篇，培养青年教师3名，培养硕士研究生1名。

本研究主要研究了和田羊和策勒黑羊血液褪黑激素水平对时空差异的响应、时空差异对和田羊和策勒黑羊组织器官中褪黑激素受体分布及表达水平的影响、和田羊和策勒羊褪黑激素与组织器官中MTR1a表达的互作机制，以及二者互作对和田羊和策勒黑羊排卵率、胚胎存活率和产羔率的影响。研究发现：和田羊和策勒黑羊Mel 的分泌存在明显的昼夜节律和季节节律，同时也存在品种之间的差异性；同时，MTRN1A基因和蛋白在策勒黑羊与和田羊的卵巢、子宫中均有表达，但是二者在不同品种间、不同组织中以及不同季节的表达量上均存在品种的差异性。本项目在研究过程中发表研究论文5篇，其中中文核心期刊4篇，普刊1篇，获批实用新型专利3个，培养青年教师3名，培养硕士研究生2名，本科生5名。本研究为阐明绵羊多胎机制和完善羊发情排卵调控理论提供依据，能够为开发诱导和田羊发情排卵新技术和提高羊繁殖力奠定理论基础，在保护地方优良品种的多样性上有着重要的意义。