载淫羊藿苷微纳羟基磷灰石/PLGA控释给药系统促进骨质疏松大鼠颅骨缺损修复的研究

Osteoporosis is a class of systemic metabolic diseases, characterized by bone mass reduced, the fine structure of the bone tissue leading to bone fragility degeneration, which is prone to fracture, resulting in the occurrence of bone defects, meanwhile osteoporosis has insufficient new bone formation and some other defects. Based on the previous study, we preferably choose hydroxyapatite and PLGA scaffolds, which have great biological properties. Furthermore, we probe the mechanism of osteoblastic differentiation of bone marrow mesenchymal stem cells stimulated by icariin and the inhibition of osteoclasts, and then adopt the potential osteogenic role of icariin to load on the micro-nano-hydroxyapatite/PLGA composite scaffolds, and then the controlled release systems were coated with bone marrow mesenchymal stem cells to build new bone tissue engineering, which was applied to the calvarial defect in osteoporosis rat and evaluate the osteogensis effects in vivo. This study provides theoretical foundation for the adhibition of traditional Chinese medicine icariin in bone tissue regeneration, meanwhile the dynamic integration of advantage of materials, cells and drug control system based on "Bone tissue engineering", which is construted by micro-nano-hydroxyapatite / PLGA controlled release system, will be conducive to the promotion of new bone formation in osteoporosis patients, acceleratting the reconstruction of chewing function, which is expected to become the new safe and effective method to promote bone defect repair in osteoporosis.

骨质疏松是一类以骨量减少、骨组织微细结构退化而导致骨脆性增加为特征的全身代谢性疾病，易发生骨折，造成骨缺损的发生，且骨质疏松患者存在着新骨形成不足等缺陷。课题组在前期工作基础上，优选具有良好生物学性能的羟基磷灰石和PLGA作为支架材料，利用淫羊藿苷潜在的促成骨作用，在深入探讨其促进骨髓间充质干细胞成骨分化及破骨抑制作用的基础上，以微纳米羟基磷灰石/PLGA复合支架材料装载淫羊藿苷，并以此控释系统，负载骨髓间充质干细胞，构建新型组织工程骨，应用于骨质疏松大鼠颅骨缺损部位，评价其体内成骨效果。本研究可为中药淫羊藿苷在骨组织功能性再生中的应用提供理论依据，而基于以微纳米羟基磷灰石/PLGA控释系统构建的“组织工程骨”将材料、细胞和药物控释的优势有机结合，将有利于促进骨质疏松患者的新骨形成，加快口腔咀嚼功能的重建， 有望成为安全高效的促进骨质疏松骨缺损修复的新方法。

骨质疏松是一类以骨量减少、骨组织微细结构退化而导致骨脆性增加为特征的全身代谢性.疾病，易发生骨折，造成骨缺损的发生。课题组在前期工作基础上，优选具有良好生物学性能的羟基磷灰石（HAp）和PLGA作为支架材料，利用淫羊藿苷潜在的促成骨作用，在深入探讨其促进骨髓间充质干细胞成骨分化及破骨抑制作用的基础上，以微纳米HAp /PLGA复合支架材料装载淫羊藿苷，并以此控释系统，负载骨髓间充质干细胞（BMSCs），构建新型组织工程骨。本项目进一步开展了微纳米HAp/丝蛋白和CPC装载淫羊藿苷，并在负载BMSCs后应用于骨质疏松大鼠颅骨缺损部位，此二种组织工程骨可有效地促进骨缺损的再生修复。本研究可为淫羊藿苷在骨组织功能性再生中的应用提供理论依据，而构建的“组织工程骨”将材料、细胞和药物控释的优势有机结合，将有利于促进骨质疏松患者的新骨形成，加快口腔咀嚼功能的重建，有望成为安全高效的促进骨质疏松骨缺损修复的新方法。本项目执行期间，发表标注本项目SCI论文2篇；参与培养硕士研究生1名、在读博士生1名。已完成计划任务书的各项考核指标。