细胞表面神经递质分布和传输的原位拉曼光谱研究

The study of the distribution, transmission and conversion of neurotransmitters on neuron is crucial and significant to the understanding of the laws governing the operation of the whole nervous system, and to the mechanism, prevention and treatment of neurodegenerative diseases. However, there exist several basic scientific questions in the in situ study of trace neurotransmitters instantaneous releasing in neuron, including the cytotoxicity of nanoparticles, the composition and distribution of neurotransmitters on neuron, the release behaviors and their correlation of different neurotransmitters, and so on. Aiming at solving these issues, we proposed a strategy to allow simultaneous real time multiplex analysis of the release behaviors of a variety of neurotransmitters molecules of PC12 cells and their correlations, the strategy is to use Shell-isolated nanoparticle-enhanced Raman spectroscopy（SHINERS）. We expect to provide important insights into the developing of SHINERS as a simple, fast, non-destructive tool used in cell research, and into the mechanism of neurodegenerative diseases.

研究神经细胞上神经递质的分布、传输和转移转化对了解神经系统运行规律，发现神经退行性疾病的发生机制进而指导其预防和治疗具有重要意义。但目前对细胞水平上痕量神经递质毫秒级释放的原位研究存在诸多问题：包括原位检测中的细胞毒性、神经细胞表面神经递质组成和分布情况、不同神经递质的释放规律及彼此间的相互关联性等问题。本项目正是基于上述问题，探索将课题组发展的壳层隔绝纳米粒子增强拉曼光谱技术用于PC12细胞的原位检测，通过研究阐明不同壳层隔绝纳米粒子对PC12细胞的生物学效应（细胞毒性），揭示细胞表面神经递质的分布情况，以及它们释放的规律及彼此间相互关联性等。期望通过本项目的研究为发展出用于细胞原位检测的拉曼光谱技术、为探明神经退行性疾病的发生机制提供理论和实验依据。

构建神经细胞上神经递质的原位检测方法，有助于其分布、传输和转移转化的了解，这对于神经退行性疾病的预防和治疗有重要意义。项目解析了不同纳米粒子对PC12细胞生物相容性的影响，并由此发现了一种具有极好生物相容性的SERS增强纳米粒子，后续将其用于PC12细胞表面神经递质组成和分布情况、不同神经递质的释放规律及彼此间的相互关联性的研究，完成了既定研究计划。项目发表标注文章5篇，申请发明专利2项。