镉胁迫下甜瓜的生理响应及野生抗镉种质的基因水平转移

Heavy metal cadmium (Cd) in the environment endangering human health through the food chain enrichment.Gansu is the hardest hit of cadmium pollution in China and it is also the advantage of the main producing areas of melon. Breeding resistance to cadmium material is of great significance for enhancing the safety of fruits and edible. The project intends to have completed the soil cadmium basic content census cadmium performance in Gansu and abtaind wild melon germplasm which anti-determination on the basis of cadmium physiological responses and differential gene expression analysis. Screened closely associated with the heavy metal cadmium resistance to gene fragments, RACE technique to obtain the full length cDNA. Further build a sense expression vector, the level of cadmium resistance genes through genetic engineering technology transfer to enhance the ability of melon anti cadmium, and at the same time build RNAi vector conversion wild germplasm inhibit expression strain, combined the plants under Cd stress response mechanism, from the reverse genetics point of view to resolve the molecular mechanisms of melon ease cadmium stress related genes. Project to improve crop resistance to cadmium by transgenic technology, to enhance the security level of production of the melon, so as to provide new ideas to solve the problem of environmental pollution of heavy metals.

环境中的重金属镉（Cd）能够通过食物链富集危害人类身体健康，甘肃是我国镉污染的重灾区，也是我国西甜瓜的优势主产区，因此选育抗镉材料对于提升瓜果食用安全具有重要意义。本项目拟在已经完成甘肃土壤镉基本含量普查和对野生甜瓜种质抗镉性能测定的基础上，通过对野生抗镉种质和栽培白兰瓜种质对重金属镉胁迫下的生理应答以及基因差异表达分析，筛选出与重金属镉抗耐性密切相关的基因片段，采用RACE技术获得基因全长。进一步构建正义表达载体，通过基因工程技术完成抗镉基因的水平转移，提升甜瓜的抗镉能力，同时构建RNAi载体转化野生种质获得抑制表达株，结合镉胁迫下植株的响应机制，从反向遗传学的角度解析甜瓜相关基因缓解镉胁迫的分子机制。项目通过转基因技术改善作物抗镉能力，提升甜瓜的安全生产水平，从而为解决我国重金属的环境污染问题提供新思路。