利用光谱移动效应对动脉硬化早期诊断的研究

Atherosclerosis (AS) is a major age-related disease and difficult to diagnose in early stage. For most patients, only if the cardiovascular complications occur, the atherosclerotic diseases will be found in early AS and treated, the damage of endothelial cells could be reversed. The researches have shown that vascular cell adhesion molecule-1(VCAM-1）is closely related with the occurrence of AS. It is possible to release the difficulty in diagnosis of early AS, as long as one can detect the low concentration of VCAM-1 in the vascular distribution. The development of quantum dots (QD) probe technology is expected to give light on this problem. In our preliminary study, we have designed and manufactured QD (VQD) combined with VCAM-1. We found that QD fluorescence peak was significantly blue-shifted about 30nm when VQD combined with VCAM-1 specifically. However, the fluorescence of VQD was too weak to accurately measure when VACM-1 content was less than 5μg/mL. In order to measure the VCAM-1 at low concentration, this project aims on the optimization of quantum dot based on VQD, to maximize blue-shift value when the QD combined with VCAM-1. This optimized QD will reduce the influence from circumstance of blood vessels on the quantum dots fluorescence properties. Through the detection of distribution of quantum dots in the blood vessels, we can get the relevance between QD fluorescence image and early vascular damage of AS, which might be useful to the early diagnosis of AS.

动脉硬化（AS）是一种衰老性疾病，早期很难被诊断，多数患者出现心脑血管并发症后才被发现，若能在早期进行诊治，可逆转受损的内皮细胞。研究表明：VCAM-1与AS的发生密切相关，如能解决低浓度VCAM-1在血管上分布，有可能形成探索AS早期诊断研究的新方法。量子点探针技术的发展有望突破这一难题。我们前期研究：设计制作了与VCAM-1结合的量子点（VQD）；当VQD与VCAM-1特异结合后，光谱出现了30nm的移动；然而当VCAM-1浓度低于5µg/mL时，由于VQD荧光太弱，干扰了检测的准确性。为了检测低浓度VCAM-1，本项目拟对前期VQD进行优化，使其与VCAM-1结合后光谱移动更大，并利用量子点光谱移动效应，使其荧光受到血管环境的干扰更小；通过量子点在血管上的分布，判断其与AS早期血管损伤间的关联性，为探索AS早期诊断方法奠定基础。

动脉硬化（AS）是一种衰老性疾病，早期很难被诊断，多数患者出现心脑血管并发症后才被发现，若能在早期进行诊治，可逆转受损的内皮细胞。研究表明：VCAM-1与AS的发生密切相关，如能解决低浓度VCAM-1在血管上分布，有可能形成探索AS早期诊断研究的新方法。量子点探针技术的发展有望突破这一难题。我们设计制作了与VCAM-1结合的量子点（VQD）；当VQD与VCAM-1特异结合后，光谱出现了30nm的移动；然而当VCAM-1浓度低于5μg/mL时，由于VQD荧光太弱，干扰了检测的准确性。为了检测低浓度VCAM-1，本项目对VQD进一步进行优化，使其与VCAM-1结合后光谱移动更大，并利用量子点光谱移动效应，使其荧光受到血管环境的干扰更小；通过量子点在血管上的分布，判断其与AS早期血管损伤间的关联性，为探索AS早期诊断方法奠定基础。