应用磁性导引骨髓间充质干细胞技术靶向治疗脊髓损伤的实验研究

BMSCs transplantation is considered to be one of the most promising treatments in the treatment of spinal cord injury. However, transplantation in situ has enough MSCs cells, it will bring the second spinal cord injury; While intravenous and subarachnoid transplantation bring small trauma, the number of MSCs migrating to the spinal cord injury cell is less, which makes curative effect limited. The project on the basis of preliminary study, we plan to design a new type of magnetic targeted cell delivery system, then we will transplant MSCs traced with SPIO into the rats' spinal cord injury model through subarachnoid. We use the characteristics of the attraction between magnetic cells and the magnet disk to enhance the specificity of MSCs' targeted migration, aiming at improving the mobility and cell number of MSCs migrating into the spinal cord injury, which be helpful for MSCs to repair spinal cord injury. This will provide a new idea and method for the BMSCs transplantation in the treatment of spinal cord injury. Meanwhile we use the molecular imaging technology of MR to observe the nest , migration and other states of MSCs in the body, then we can obtain the trace imaging of the transplanted stem cells in vivo. This technology can overcome the limitation of the traditional trace method which only can analysis in vitro, and can help us observe the distribution and live condition of the transplanted stem cells dynamically and real-time. Meanwhile it has the important meaning to evaluate the effect of stem cell transplantation and optimize the scheme of SCI treatment using the stem cells transplantation.

骨髓间充质干细胞(MSCs)移植治疗脊髓损伤被认为是最有前景的治疗方法之一。然而，原位移植会造成脊髓损伤区的二次创伤，静脉移植和蛛网膜下腔移植创伤小，但迁徙到损伤区的细胞数较少，疗效受限。 本项目在前期研究基础上，拟设计一种新型的磁性靶向投递系统，通过蛛网膜下腔移植磁标记MSCs到脊髓损伤模型内，并在损伤水平外置磁场，利用磁标细胞与磁铁相互吸引的特点，增强MSCs靶向迁徙的特异性，旨在提高MSCs靶向脊髓损伤区的迁移率及细胞数量，明显改善脊髓损伤的功能恢复，为MSCs移植治疗SCI提供了一种新的思路和手段。同时利用MR分子成像技术，观察MSCs在体内的归巢、迁徙等的情况，实时、动态示踪移植细胞，从而揭示磁靶向投递系统对移植细胞分布、迁徙及归巢影响的动力学规律，为MSCs移植治疗脊髓损伤提供了一种新的思路和手段，对于评估MSCs移植效果及优化MSCs治疗脊髓损伤方案有着重要意义。

骨髓间充质干细胞(MSCs)移植治疗脊髓损伤被认为是最有前景的治疗方法之一。然而，原位移植会造成脊髓损伤区的二次创伤，静脉移植和蛛网膜下腔移植创伤小，但迁徙到损伤区的细胞数较少，疗效受限。. 本项目在前期研究基础上，设计了一种新型的磁性靶向投递系统，通过蛛网膜下腔移植磁标记MSCs到脊髓损伤模型内，并在损伤水平外置磁场，利用磁标细胞与磁铁相互吸引的特点，增强MSCs靶向迁徙的特异性，提高了MSCs靶向脊髓损伤区的迁移率及细胞数量，明显改善脊髓损伤的功能恢复，为MSCs移植治疗SCI提供了一种新的思路和手段。同时利用MR分子成像技术，观察MSCs在体内的归巢、迁徙等的情况，实时、动态示踪移植细胞，从而揭示磁靶向投递系统对移植细胞分布、迁徙及归巢影响的动力学规律，为MSCs移植治疗脊髓损伤提供了一种新的思路和手段，对于评估MSCs移植效果及优化MSCs治疗脊髓损伤方案有着重要意义。