卵白蛋白/多糖双功能复合胶体颗粒构筑及乳液输送特性调控机制研究

Biological active ingredients are important nutrients for human body, the main difficulty remains now is their low bioavailability. It has been known that the carrier system structure is a key factor affecting the bioavailability, however, the structure-activity relationship between the carrier and the active ingredient it carries is still lack of in-depth research.. In this study, we use ovalbumin and polysaccharide (Gum arabic and Carboxymethyl chitosan) to prepare ovalbumin/polysaccharide composite colloidal particles by complex-coacervation and thermal induction, and find out the formation mechanism and the critical control elements of the compound colloid particles. And then, dual-function composite colloid particles (Antioxidant and interface stability function) which loaded with curcumin will be prepared, and used to establish dual-functional composite colloidal particles emulsion delivery systems, and the purpose is to find out the mechanism of the dual functional composite colloidal particles in the oil-water interface. Then via in vitro digestion and Caco-2 cells absorb model, the inter relevance of dual-functional composite colloidal particles, the interface structure of emulsion, and the active ingredient in controlled release characteristics will be studied, as well as the regulatory mechanism of the emulsion delivering character of the composite colloidal particles. The project will realize efficient delivery and synergistic effect of active ingredients, and provide new ideas and theoretical guidance for the design, preparation and research of functional emulsion delivery systems.

生物活性成分是人体重要的营养素，其生物利用率低是目前存在的主要问题。其中载体系统结构是影响其生物利用率的关键因素，然而对载体结构与所携载活性成分之间的构效关系仍缺乏深入的研究。. 本项目以卵白蛋白和多糖（阿拉伯胶和羧甲基壳聚糖）为研究对象，利用复合凝聚法与温和热协同构筑卵白蛋白/多糖复合胶体颗粒，确定复合胶体颗粒形成机制和关键控制因子；在此基础上进一步制备负载姜黄素的卵白蛋白/多糖双功能复合胶体颗粒（抗氧化和界面稳定功能），并以双功能复合胶体颗粒为乳化剂制备鱼油乳液，揭示双功能复合胶体颗粒在油水界面作用机制，结合体外模拟消化和Caco-2细胞吸收模型，阐明双功能复合胶体颗粒、乳液界面结构、活性成分控释特性三者的内在联系，揭示复合胶体颗粒对乳液输送特性的调控机制，最终实现活性成分稳定、高效输送及功能协同效应，为功能化乳液输送系统的设计、制备与研究提供新思路和理论指导。

采用复合凝聚法和温和热诱导协同制备了卵白蛋白和多糖复合胶体颗粒，研究了卵白蛋白与多糖的相互作用的影响因素，考察了制备条件对复合胶体颗粒尺度、表面电荷、形貌等理化特征的影响，探讨复合凝聚物形成的驱动力和相分离机制，以卵白蛋白/多糖复合胶体颗粒为载体制备了负载姜黄素的颗粒，研究双功能复合胶体颗粒对姜黄素的负载率、保留率及稳定特性，从热力学和动力学的角度证明了姜黄素在复合胶体颗粒内外部的正确分布形式。.以卵白蛋白/多糖复合胶体颗粒为乳化剂制备MCT乳液，研究了不同条件下制备的复合胶体颗粒的几何形状和刚性/柔性等对乳液液滴大小、界面吸附、微观结构及稳定性的影响，发现除颗粒湿润性外复合胶体颗粒的刚性和柔性在界面的吸附机制中的重要作用，并以负载姜黄素的胶体颗粒制备了双功能乳液系统，对乳液在环境条件下的理化性质和功能特性进行系统的表征，分析双功能颗粒的抗氧化特性能，明确卵白蛋白/多糖双功能复合胶体颗粒在油水界面吸附稳定机制。.进一步构建了负载鱼油的乳液，通过体外模拟法研究乳液载体在人体内的消化行为，利用动态光散射和Zeta电位测定乳液在模拟胃肠液中粒径、分散系数、表面电荷的变化，同时结合显微镜对乳液液滴的形貌特性进行观察，分析DHA的包埋率，稳定性和利用率，解析了复合胶体颗粒特征与活性物质包埋释放之间的关联，该项目实现活性成分稳定、高效输送及功能协同效应，为功能化乳液输送系统的设计、制备与研究提供新思路和理论指导。