猪亲本及其杂种DNA甲基化差异与杂种优势的关系
基本信息
结项摘要
构建梅山猪、大白猪和长白猪的3个亲本群（梅山猪×梅山猪、大白猪×大白猪、长白猪×长白猪）和4个正反交的杂交组合（大白猪×梅山猪、梅山猪×大白猪、大白猪×长白猪、长白猪×大白猪），采用高效液相色谱仪测定猪杂种与亲本血液和肌肉组织中DNA甲基化水平，分析DNA甲基化水平与杂种优势的关系；采用以亚硫酸盐（bisulfite）处理DNA为基础的甲基化敏感的单链构象分析（MS－SSCA）法研究猪杂种与亲本间特异功能基因或调控基因启动子区DNA甲基化模式，探讨甲基化差异与基因表达差异或杂种优势的关系，从DNA甲基化差异的角度揭示杂种优势产生的分子遗传学机理。该项目将为猪杂种优势的遗传机理提供分子证据，具有重要的理论意义。
项目摘要
项目成果
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数据更新时间：2023-05-31
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