典型环境激素在好氧颗粒污泥污水处理过程中的毒性效应、作用机制及行为归趋

Environmental hormone compounds are latent, persistent and bioaccumulation, which have attracted considerable attention as one of emerging pollutants around the world. The objective of this project is to investigate the toxicity, mechanism and behavior of typical environmental hormones in aerobic granular sludge treatment process. Firstly, the wastewater treatment efficiency and related mechanism will be invested in the presence of environmental hormone compounds in aerobic granular sludge system by using the combination of macro- and micro analysis. According to comprehensive utilization of a series of spectral techniques, the formation characteristics, chemical structure and released performance of soluble microbial products in aerobic granular sludge system will be studied. Next, the sorption and degradation of environmental hormone compounds will be analyzed, especially with respect to the role of extracellular polymeric substances and their interaction with environmental hormone compounds. In the end, the population structure and biological diversity of aerobic granular sludge will be investigated in the exposure of environmental hormone compounds by using various modern molecular biological techniques. Based on the results of this project, it is helpful to fully reveal the potential risks and environmental impact of toxic compounds in wastewater treatment process, and also give information to further understand and improve the treatment effect of these pollutants.

环境激素类化合物具有潜伏性、持久性和生物富集性等特点，是国内外广受关注的新兴污染物之一。本项目旨在探明典型环境激素在好氧颗粒污泥处理系统中的毒性效应、作用机制及行为归趋。通过宏观检测和微观分析相结合的手段，明确环境激素化合物的存在对好氧颗粒污泥污水处理效率综合影响及相关致毒机制；利用光谱分析技术与手段，探讨环境激素胁迫下好氧颗粒污泥溶解性有机物（SMP）形成特性、化学结构及释放规律；分析环境激素在好氧颗粒污泥处理过程中的吸附与降解规律，重点考察胞外多聚物（EPS）对环境激素化合物去除所发挥的贡献与作用，明确EPS与环境激素的相互络合机制；借助各种现代分子生物学技术手段，考察环境激素长期暴露下好氧颗粒污泥种群结构和生物多样性的演变规律。基于本项目的研究成果，有助于全面揭示有毒有害化合物在污水处理过程中的潜在风险和环境影响，同时对进一步认识、改善和提高这些污染物的治理效果具有重要的指导意义。

环境激素作为具有高毒性、生物累积性的难降解有机污染物，在污水处理系统中检出率越来越高，对生态环境的危害日益严重。本项目选取典型环境激素类化合物（2,4-DCP、2,6-DCP、环丙沙星等），明确了环境激素对好氧颗粒污泥的生物毒性效应；综合利用三维荧光、平行因子分析、同步荧光光谱、二维相关光谱和凝胶色谱等一系列光谱测试及分析手段，多角度分析了典型环境激素胁迫下的好氧颗粒污泥EPS和SMP释放特性；针对环境激素难降解问题，开发和拓展了一系列以好氧颗粒污泥为核心的污水处理工艺，建立了共代谢、芬顿预处理、MFC强化处理等一系列环境激素类协同强化处理技术，明确了好氧颗粒污泥反应器最佳运行参数；结合高通量测序技术，阐明了系统微生物优势种群结构，探明了污染物去除效果和作用机制。针对环境激素的检测问题，进一步开展了一系列高灵敏的传感方法的建立。已在Biosensors and Bioelectronics、Bioresource Technology等学术期刊发表SCI收录论文20余篇，授权国家发明专利4项，培养博士硕士研究生共10人，取得了良好的研究效果。