ABA促进植物蜕皮激素合成的生理与分子机制研究

The project is based on the skilled technology of regulating Ajuga suspension cells for high yield of phytoedysteroids in the former stage. It is founded that abscisic acid (ABA) significantly stimulated the synthesis of phytoedysteroids, but its regulation mechanism of synthesis phytoedysteroids is still not clear. Therefore, techniques such as plant physiology and biochemistry, molecular biology, mass spectrometry detection were intent to be applied in this project, the relationship between the start-up of Ajuga cells defense system induced by ABA and synthesis of phytoedysteroids was studied as well as the relationship between the change of plant endogenous hormones of Ajuga cells induced by ABA and the synthesis of phytoedysteroids; technologies of transcriptomics and metabonomics were utilized synthetically to explore key genes and metabolites of ABA which induced the synthesis of phytoedysteroids and its function was also verified. So far there are no reports on this study at home and abroad, it will enrich the theory of hormone's controlling on secondary metabolite and provides theoretical basis and technical support to taking advantage of cells for mass production of phytoedysteroids.

本项目是在前期调控筋骨草悬浮细胞高产植物源蜕皮激素的成熟技术基础上提出的。发现外施脱落酸（ABA）显著激发了植物源蜕皮激素的合成，然而其调控蜕皮激素合成的机理还不清楚。为此，本项目拟运用植物生理生化、分子生物学、质谱检测等技术，研究ABA诱导筋骨草细胞防御系统启动与蜕皮激素合成的关系；ABA诱导筋骨草细胞内源激素的变化与蜕皮激素合成的关系；综合利用转录组学技术和代谢组学技术发掘ABA诱导蜕皮激素合成的关键基因和代谢物，并对其功能进行验证。国内外尚无关于本研究的报道，其开展将充实激素对次生代谢产物的调控理论，同时为利用细胞大规模生产植物源蜕皮激素提供一定的理论依据和技术支持。

本项目将ABA作为调控激素研究其诱导筋骨草悬浮细胞合成蜕皮激素的机制。实验发现ABA诱导20E含量增加，但生长激素GA3、ZR、IAA含量下降，这与生长素类激素加快细胞生长及分裂而引起的次生代谢产物增加的作用无关。细胞内SOD、CAT以及POD活性升高，说明ABA除介导细胞依次发生信号识别和传导作用外，对植物细胞也有一定的胁迫作用。经ABA诱导，与蜕皮激素合成相关的ko00900萜类合成主干通路有6个基因差异表达，ko00100甾体合成通路有4个基因差异表达，ko00140甾体激素生物合成通路有6个基因差异表达。本项目的研究成果充实了激素对次生代谢产物的调控理论，为后续利用细胞大规模生产植物源蜕皮激素研究提供一定的理论依据和技术支持。