相分离载体溶胶、凝胶分子印记新技术及其仿生识别应用

This project is to study the new technology of sol-gel molecular imprinting with phase separating polymer-immobilized templates in order to overcome the difficulty in molecular imprinting of biomacromolecules and find its application in mimetic molecular recognition of proteins and nucleic acids. A molecular imprinting technique of biomacromolecules has been preliminarily established with a reversed-phase micellar system to prepare a sol-gel molecule-imprinted, inorganic-organic hybrid SiO2 materials. Meanwhile, the "artificial-antibody" nanoparticles prepared with this technique were also applied to the biomimetic fluorescence immunoassay of human IgG. Although a low specificity of recognition is gained in the result due to the nonspecific adsorption of this kind of materials, this research provides a valuable way for the molecular imprinting of biomacromolecules and, therefore, is still important in academic significance.

研究相分离载体溶胶-凝胶分子印记（MIG）新技术，突破生物大分子分子印记的难题，制备新型的MIG材料并考察其理化性质；结合新型荧光探针技术，建立目标分子的高特异性仿生肿邮侗鹩胗饧觳庑路椒ǎ谎芯縈IG模拟酶的分析应用；探索MIG材料在仿生传感器方面的τ谩８醚芯吭谏蒲А⒒肪晨蒲У攘煊蛴兄匾蒲б庖搴陀τ眉壑怠