铜硫硒纳米结构用于白血病肿瘤诊疗过程中的实时监控研究

Introducing the designed nano-reagent into tumor for real-time monitoring the complex biochemical system analysis is benefit to the precise diagnosis and treatment of disease. As the interaction system between the nanoprobe and tumor cells is very complex, it is difficult to acquire the qualitative and quantitative information of the interaction. This program is to develop bright near-infrared luminescent probes of nonstoichiometric copper chalcogenide nanostructures, which can serve as an efficient catalyst for click reaction for its high copper deficiency density. And it provides the possibility to functionalization of nanostructures for efficient leukemia cell targeting and imaging. Acquisition and qualitative analysis of high-throughput assays for multi-way imaging data will be done by chemometrics and computer tools, which can diagnose the leukemia tumor scientifically. Then, the real-time monitoring of imaging and therapeutic action will be used to guide the amount and way of the nanomaterials for thermal treatment of tumors. The project combines the theory and experiment to develop a promising mode for the integration of diagnosis and treatment in a multidisciplinary way. This research has both theoretical innovations and applicable potentials, which is of benefit not only to biochemical analysis, but also to the relative research fields such as biomedicines, cytobiology, molecular imaging.

将纳米试剂靶向导入肿瘤区域进行诊疗的实时监控对实现疾病精准诊断和治疗有重要意义。针对纳米试剂与肿瘤细胞相互作用的体系复杂、难以实时动态提取相互作用的定性和定量信息的问题，本项目拟构建近红外高量子产率的铜硫硒纳米结构，借助于其高载流子浓度能高效催化点击化学的特性实现原位功能化，从而靶向白血病肿瘤细胞成像；并借助化学计量学方法和计算机技术对高通量实时成像数据进行定性、定量分析，实现对肿瘤细胞的科学诊断；再结合治疗过程中的实时动态成像数据，建立光热效应模型，合理使用纳米材料，达到对肿瘤细胞精准治疗的目的。项目采用理论和实验相结合的方式，多学科交叉，建立了一种对白血病肿瘤细胞诊疗一体化实时监控与分析的模式，既有理论创新，又有实用价值，不仅对复杂体系的生化分析，而且对纳米医学、细胞生物学、影像学的相关研究都具有十分重要意义。

获取高亮度的近红外发光纳米探针对疾病诊断和治疗有重要的意义。本项目合成了一系列近红外高量子产率的铜硫硒纳米结构，实现了其在生物医学方面的应用。按照预期计划，项目取得了如下进展：.（1）构建了一系列近红外区高发光效率的非计量比的Cu2-xSySe1-y纳米结构，探讨了反应机理。.（2）对Cu2-xSySe1-y发光探针进行功能化，对癌细胞的靶向成像，联合化学计量学方法，实现了成像信息的有效提取；.（3）建立Cu2-xSySe1-y发光探针和周围环境热量传导的光热效应模型，结合光热过程中的实时成像分析，指导合理使用Cu2-xSySe1-y进行光热治疗。