大丽轮枝菌微菌核萌发的分子调控机理研究

Verticillium dahliae is one kind of soilborne plant pathogens which can infect 184 crops and cause Verticillium wilts.Verticillium wilt of cotton became a serious problem after cotton bollworm in world cotton production. Microsclerotium is the main survival structure of V. dahliae in soil, and the inoculum of primary infection of Verticillium wilt. It can survive for 14 years in soil. The incidence and severity of Verticillium wilt are determined by the quantity and survival status of microsclerium in soil.In order to understand molecular regulating mechnisms of microsclerotium domancy and germination, this research will fucos on transcriptome change of microsclerotium from domancy to germination using defoliating pathotype of V. dahliae strain XJ2008 based on our previous results of the production, collection, and RNA extraction of microsclerotium. The technologies and methods of RNA-seq, bioinformatics, gene knock out, and function complementary will be used to get RNA sequences, to compare the dynamic change of transcriptome, to clone the differential expressing genes, to analyze the signal pathway and gene regulation, to confirm the function of those key genes during microsclerotium germination.This research will make a base for establishing a new method of Verticillium wilt controling from the point of regulating microsclerotium dormancy, germination, and survival.

大丽轮枝菌是一种土传植物病原真菌，可侵染184种农作物引致黄萎病。其中棉花黄萎病危害尤为严重，由于缺乏抗病种质资源，已成为继棉铃虫之后的又一个世界性难题。微菌核是大丽轮枝菌在土壤中的主要存活结构和黄萎病的初侵染菌源，可以在土壤中存活长达14年之久，其数量及存活状况决定了黄萎病的发病率和危害程度。本研究拟在前期建立的微菌核产生、收集及RNA提取技术的基础上，以落叶型大丽轮枝菌XJ2008菌株为研究对象，采用RNA-seq、生物信息学、基因敲除及功能验证等技术和方法，比较微菌核休眠至萌发过程中转录组水平变化，分析信号传递通路和基因调控规律，克隆关键调控基因并研究其功能，揭示大丽轮枝菌微菌核休眠至萌发的分子调控机理，为从调控微菌核的休眠、萌发及存活角度，建立黄萎病防治新方法奠定理论基础。

大丽轮枝菌是一种土传植物病原真菌，可侵染184种农作物引致黄萎病。其中棉花黄萎病危害尤为严重，由于缺乏抗病种质资源，已成为继棉铃虫之后的又一个世界性难题。微菌核是大丽轮枝菌在土壤中的主要存活结构和黄萎病的初侵染菌源，可以在土壤中存活长达14年之久，其数量及存活状况决定了黄萎病的发病率和危害程度。本研究在前期建立的微菌核产生、收集及RNA提取技术的基础上，以落叶型大丽轮枝菌XJ2008菌株为研究对象，采用Illumina高通量测序技术、生物信息学、基因敲除及功能验证等技术和方法，比较微菌核休眠至萌发过程中转录组水平变化，分析信号传递通路和基因调控规律，克隆关键调控基因并研究其功能，揭示大丽轮枝菌微菌核休眠至萌发的分子调控机理，为从调控微菌核的休眠、萌发及存活角度，建立黄萎病防治新方法奠定理论基础。