多环芳烃生物降解的动力学研究

In this project, both the production of lignin peroxidase and manganese peroxidase by Phanerochaete chrysosporium as well as the production of laccase by Coliolus versicolor were studied. During the production of lignin peroxidase and manganese peroxidase by Phanerochaete chrysosporium fermentation, benzyl alcohol was firstly suggested to replace veratryl alcohol to induce the expression of enzymes. The immobilization of Phanerochaete chrysosporium with polyurethane particles and self-immobilized Coliolus versicolor were adopted for repeated-batch or continuous production of enzymes, respectively. The biodegradation of dyestuffs and wastewater from dyeing and printing industry were evaluated by lignin peroxidase, laccase and mycelium respectively. The operation conditions were optimized and the biodegradation kinetics were proposed. Either enzymes or mycelium exhibited high capability to biodegrade dyestuffs and wastewater.

利用白腐真菌产木质素酶，再进一步催化多环芳烃的降解、对于含难降解、重污染的工业废水治理具有重要的理论意义和应用前景。本项目通过诱导物的筛选，消除产酶对黎芦醇和纯氧的依赖性，通过将产酶和多环芳烃的降解在一个生物反应器内同步完成，消除降解对双氧水的依赖。通过细胞固定化新型生物反应器研究及产酶和降解动力学的研究优化整个过程。