甘蓝型油菜有限花序种质创制及其遗传分析

The unlimited inflorescence of rapeseed increased invalid consumption of biomass and influenced potential of seed yield per plant. And rapeseed have long flowering and silique mature inconsistencies, which affected its mechanizing harvesting. Therefore, discovery and creation of new materials with determinate inflorescence is one of important approaches of alleviating these problems. In this project, we intend to make interspecific hybridization between B. napus and B. carinata to create new materials with determinate inflorescence, and try to obtain a new way of transfering determinate inflorescence genes of B. carinata and a number of genetic stability of B. napus new materials with determinate inflorescence. We intend to make genetic analysis of different generations in the process of creating determinate inflorescence materials by GISH/FISH technology. And we also make genetic analysis of determinate inflorescence character to mapping the determinate inflorescence genes by means of molecular maker technology, which could provide theory basis for genetic improvement, clone and functional study of the determinate inflorescence gene in B.napus.

油菜的无限花序增加了生物量的无效消耗，影响了单株产量潜力的发挥，且花期长，角果成熟不一致，影响了机械化收获。因此，发掘和创制有限花序新材料，是缓解上述问题的重要途径之一。本研究利用甘蓝型油菜与埃芥种间杂交开展有限花序新材料的创制，探讨建立一套转移埃芥有限花序基因的新方法，获得一批遗传稳定的甘蓝型油菜有限花序新材料，利用GISH/FISH技术对有限花序新材料创制过程中的不同世代材料进行遗传分析，完善其细胞学基础，并利用分子标记技术开展有限花序性状的遗传分析，实现有限花序基因的定位。为油菜有限花序遗传改良及其基因克隆与功能研究奠定基础。

油菜的无限花序增加了生物量的无效消耗，影响了单株产量潜力的发挥，且花期长，角果成熟不一致，影响了机械化收获。因此，发掘和创制有限花序新材料，是缓解上述问题的重要途径之一。本研究利用甘蓝型油菜与埃芥种间杂交开展有限花序新材料的创制，探讨建立一套转移埃芥有限花序基因的新方法，获得一批遗传稳定的甘蓝型油菜有限花序新材料，利用GISH/FISH技术对有限花序新材料创制过程中的不同世代材料进行遗传分析，完善其细胞学基础，并利用分子标记技术开展有限花序性状的遗传分析，实现有限花序基因的定位。为油菜有限花序遗传改良及其基因克隆与功能研究奠定基础。