玉米响应高温胁迫的DNA甲基化调控及抗热性机理研究

Recently, the mechanism of stress-induced epigenetic regulation and resistance in plants is one of the hot spots of stress molecular biology research, significantly in both theory and practice. In this project, genome-wide identification and characterization of expression profiling of heat resistance and epigenetic regulation response genes and DNA methylation analysis of related candidate genes from the maize B73 line under the condition of heat stress will be performed through the methods of transcriptomics and epigenomics, respectively. On this basis, the relationship between expression profiling and DNA methylation variations of the heat resistance response genes will be analyzed and also the mechanism that the changes of expression profiling of epigenetic regulation response genes have an influence on expression profiling and DNA methylation variations of the heat resistance response genes will be revealed. The function of some important stress-induced response genes will also be screened and identified in order to probe the regulation approach and mechanism of DNA methylation in response to heat stress and further reveal the mechanism of stress-induced response and heat resistance regulation in maize. This study may provide new ways and means to study the molecular regulation mechanism and epigenetic improvement of plant resistance.

植物在逆境胁迫下的表观遗传调控机制及抗逆性机理的研究是近年来逆境分子生物学研究的热点之一，有重要的理论和实践意义。本研究结合前期研究基础，分别采用转录组学和表观组学方法鉴定和分析高温胁迫条件下玉米B73自交系响应高温胁迫的抗热应答基因与表观遗传调控应答基因的表达量变化和全基因组范围与高温胁迫响应相关候选基因的DNA甲基化的变异情况。在此基础上，分析玉米抗热应答基因的表达谱变化与其DNA甲基化变异之间的关系以及高温胁迫下DNA甲基化调控相关表观遗传调控应答基因在转录水平的表达量变化对抗热应答基因DNA甲基化变异和表达量变化的影响，筛选与鉴定相关应答调控基因功能，探讨玉米响应高温胁迫的DNA甲基化调控途径及其作用机制，为进一步揭示高温胁迫下玉米DNA甲基化变异在响应高温胁迫的应答机制和抗热性机理方面提供重要的理论依据。该研究结果将为植物抗逆性的分子调控机制和表观遗传改良研究提供新的方法与途径。

植物在逆境胁迫下的表观遗传调控机制及抗逆性机理的研究是近年来逆境分子生物学研究的热点之一，有重要的理论和实践意义。本项目利用转录组测序鉴定与分析了玉米响应高温胁迫下1857个差异表达基因（DEGs），进一步通过KEGG通路分析发现内质网蛋白加工通路与热应激反应密切相关，167个与玉米的热激反应密切相关的转录因子可能在提高玉米抗热胁迫中起重要作用；通过全基因组DNA甲基化测序分析发现了325个差异甲基化基因(DMGs)位点的DNA甲基化变异与抗热密切相关；进一步通过对抗热应答基因与表观遗传调控应答基因的表达谱变化与DNA甲基化变异的相关性分析，初步探讨了玉米响应高温胁迫的DNA甲基化调控途径及其作用机制，为进一步揭示高温胁迫下玉米DNA甲基化变异在响应高温胁迫的应答机制和抗热性机理方面提供了重要的理论依据。此外,该研究结果丰富了植物抗逆性的分子调控机制，为植物表观遗传改良研究提供了新的方法与途径。