β-受体激动剂亲水性分子印迹聚合物识别消除样品基质效应研究

β-agonists are a group of synthetic compounds which were misused illegally in breeding of animal including swine, sheep and cow, because they act as growth promoters and also transferring fat to muscle. It is necessary to develop sensitive, accurate and robust analytical technologies to monitor the illegally misused of β-agonists in the animal breeding. However, it is a great challenge for accurate and precise determination of β-agonists in complex sample by using liquid chromatography coupled tandem mass spectrometry due to the sample matrix effect interference. Moreover, the common sample preparation technologies is too time-consuming and poor selective to decrease or eliminate the sample matrix interferences. In this study, the mechanism of sample matrix effects will be examined to quantify the matrix effect and find the main influence factor of matrix interference. The class specific and hydrophilic molecularly imprinted polymers (MIP, such as microspheres, membranes and magnetic beads) would be prepared based on synergistic effect of hydrogen bonding, ∏-∏ hydrophobic interaction and steric hindrance by optimizing the polymerization system of MIP. The developed MIP could selectively extract more than ten kinds of β-agonists in complex sample matrix. The sample matrix effect could be decreased or eliminated by good using of excellent selectivity and powerful enrichment of MIP to improve the sensitivity, accuracy and robust of the developed testing method. The results of this study could provide the powerful technical support for the accurate and precise determination of β-agonists in complex sample matrix.

β-受体激动剂（"瘦肉精"）具有机体营养再分配和促进动物生长的功效，但易在组织内残留，严重影响动物源性食品的安全。液相色谱-串联质谱（LC-MS/MS）确证分析复杂样品基质中β-受体激动剂时，样品基质效应对检测结果准确性和精密度的影响是一个巨大挑战。常规前处理手段操作复杂，样品基质效应消除效果较差。本项研究拟通过研究LC-MS/MS检测β-受体激动剂的基质效应形成机制，量化样品基质效应影响，发现基质效应产生的主要因素；基于氢键、π-π疏水作用及空间位阻协同作用，优化分子印迹聚合体系，获得高度类特异性亲水性分子印迹聚合物（微球、膜以及磁珠等），对10种以上β-受体激动剂具有识别吸附能力；基于分子印迹聚合物的高选择性和强富集能力，降低或消除样品基质效应干扰，提高复杂样品基质中痕量β-受体激动剂检测的灵敏度和准确性，为动物养殖过程和动物源性食品中β-受体激动剂精确检测提供技术保障。

beta-受体激动剂是一类饲料中非法添加物，对动物养殖和畜产品质量安全产生恶劣影响。本课题从beta-受体激动剂样品基质干扰和分子印迹样品前处理入手，开展了样品基质效应消除和系列分子印迹材料的研究。通过本课题研究，探明了饲料样品基质对-受体激动剂基质效应作用，表明饲料样品中微量元素和水溶性蛋白是对beta-受体激动剂检测过程中干扰的主要因素；以苯乙醇胺A和莱克多巴胺为模板，合成了分子印迹膜（MIM）、分子印迹微球（MIMPs）和金属有机框架分子印迹聚合物（M-MOF-MIPs）等系列分子印迹聚合物材料（MIPs），对合成的MIPs进行表征，考察了其吸附效率、选择性和净化性能等关键指标，并结合液相色谱-串联质谱建立了分析方法。所建立的方法的回收率均优于70%，相对标准偏差低于12.0%，能够满足对于饲料、动物组织和动物尿液中β-受体激动剂的控制要求。本课题产生的分子印迹材料具有较为广阔的实际应用前景，能够推动我国样品前处理技术的进步。本课题执行后，申请专利2项，获得国家发明专利授权2项，发表相关研究论文8篇，其中SCI收录8篇；获得北京市科学技术成果二等奖1项（排名第三），在国内和国际会议上做相关学术报告各1次。