姜氏菌新资源的挖掘及基于全基因组信息的生物勘探

Actinobacteria, as one of the largest bacterial phyla, are well known for their great potential to produce metabolite with bioactivity. Among them, limited studies have been done on secondary metabolites produced by the rare taxa living in unique environments, such as the group Jiangellales. The project plans to do an extensive study on the group Jiangellales and aims to establish a serial of selective isolation methods on the basis of their whole genome data and experience of our group over the past years on the study and isolation of actinobacteria living in extreme environments. The taxonomic status of the Jiangellales will be confirmed by using polyphasic approach and genome information. The secondary metabolism-related genes would be analyzed by genome mining of the group, followed by screening of bioactivities of positive strains. The potential to produce bioactive metabolites of the Jiangellales will be assessed to explore the valuable products of secondary metabolism. This project intends to solve the problem of isolation of rare actinobacteria and, thereby, accelerate the development of these rare taxa. Meanwhile, it would help researchers to confirm the taxonomic status of Jiangellales. Furthermore, this study will let us assess the bioactive metabolites potential of this taxa, and hope to obtain valuable secondary metabolite products.

放线菌作为人类生产和生活极为密切的微生物类群，其强大的代谢活性为人们所共识。然而，对于姜氏菌这一盐碱环境独特的稀有放线菌类群次级代谢产物的研究少有报道。本项目拟以姜氏菌为研究对象，在过去多年研究极端环境放线菌分离方法的基础之上，结合基因组数据，摸索一套适用于该类群放线菌的分离方法；利用现代的多相分类手段，以及全基因组信息来准确确定该类群的精确分类地位；通过对姜氏菌全基因组信息的挖掘，深入解析其次生代谢的相关基因，并结合活性筛选结果，综合评价姜氏菌产物活性次级代谢的潜力，探索有价值的次级代谢产物。通过本项目的实施，有望解决稀有放线菌分离比较困难、物种资源相对匮乏的局面，确定姜氏菌在放线菌中所处的进化地位。此外，本项目将会对姜氏菌的次级代谢潜能做出全面评估，期望挖掘有价值的活性代谢产物。

放线菌作为人类生产和生活极为密切的微生物类群，其强大的代谢活性为人们所共识。然而，对于姜氏菌这一盐碱环境独特的稀有放线菌类群次级代谢产物的研究少有报道。本项目以姜氏菌为研究对象，在过去多年研究极端环境放线菌分离方法的基础之上，结合基因组数据设计培养基，新分离出6株姜氏菌类群，且包含一个潜在新种；利用16S rRNA基因和全基因组信息来准确确定该类群的精确分类地位；通过对姜氏菌全基因组信息的挖掘，深入解析其次生代谢的相关基因，并结合活性筛选结果，综合评价姜氏菌产物活性次级代谢的潜力，并发现有潜在应用价值的姜氏菌菌株。通过本项目的实施，开拓了一条以基因组信息与分离经验相结合的微生物分离策略，有望解决稀有放线菌分离比较困难、物种资源相对匮乏的局面；采用基因组系统学的方法对放线菌系统学进行重新整理，并确定姜氏菌在放线菌中所处的进化地位，为今后放线菌研究奠定了坚实的分类学基础。此外，本项目对姜氏菌的次级代谢潜能的全面评估，将有助于后期对姜氏菌应用价值的挖掘。