端粒酶反义DNA/Arabinogalactan偶联物靶向抗肝癌实验
基本信息
结项摘要
建立了TRAP-ELISA法检测了15株消化系肿瘤细胞，13株端粒酶阳性（包括肝癌细胞株SMMC-7721、BEL-7402），表明绝大多数肿瘤细胞表达端粒酶活性。用此方法检测良恶性腹水端粒酶活性，发现肝硬化、结核性腹膜炎腹水脱落细胞不能检测端粒酶活性，而癌性腹水（肝癌、胃癌、结肠癌、胰腺癌、卵巢癌）85.29%端粒酶阳性，特异性和敏感性优于腹水AFI、CEA、CA系列，敏感性高于腹水细胞学检查，端粒酶活性检测在良恶性腹水鉴别诊断中具有较高的临床价值。设计合成针对端粒酶RNA成分的反义DNA，观察到该反义DNA可以显著抑制肝癌细胞端粒酶活性并抑制肝癌细胞增殖，诱导细胞凋亡，此作用与三氧化二砷抗肝癌作用的协同反应。
项目摘要
项目成果
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数据更新时间：2023-05-31
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