钛材料表面微纳米图案化构建及其与单细胞相互作用机制研究

It is great significant for Single-cell analysis, for which can provide the intracellular material and the intracellular biochemical reaction of the accurate information, and reflect the relationship between cell and the chemical composition at the cell and molecular level research. micro-, nano-patterned structures on titanium surface were prepared using surface modification. Interface interaction model of titanium surface micro-, nano-patterning / single-cell cell based microfluidic droplets system were constructed in vitro system. The analysises of the intracellular material and the intracellular biochemical reaction on titanium surface with micro/nano-patterned structures were carried out. Researches on influence of micro-nano-patterned structures on cell proliferation, differentiation were investigate. The mechanism of surface properties of titanium and cell interaction were also revealed. Which could provide a theoretical basis for clinical to design and prepared titanium-based bone repair materials with excellent biocompatibility.

单细胞的分析能提供出细胞内物质及细胞内生化反应的准确信息，反应出细胞的功能与化学组分间的特定关系，在细胞和分子水平上进行研究材料/单细胞之间相互作用机理具有重要意义。本项目在钛表面构建表面微、纳米图案化结构并赋予特定区域功能化，基于微流控液滴系统在体外构建钛表面微纳米图案化/单细胞界面相互作用模型，系统研究单细胞在不同微纳米图案化结构的钛基骨修复材料表面的细胞内物质及细胞内生化反应规律，研究钛表面微纳米图案化结构对单细胞和细胞群的增殖、分化的影响规律，揭示钛基骨修复材料表面特性与细胞相互作用机制，为临床构建出生物相容性优异的钛基骨修复材料提供理论依据。

本项目在钛表面分别构建导电高分子纳米结构，电化学沉积钙磷涂层，实现了钛表面的功能化，并实现结构的微图案化，赋予特定区域功能化，在体外构建钛表面微纳米图案化/细胞界面相互作用模型，系统研究细胞在不同微纳米图案化结构的钛基骨修复材料表面的细胞内物质及细胞内生化反应规律，研究钛表面微纳米图案化结构对细胞的增殖、分化的影响规律。结果表明，钛表面的微电场图案结构能为细胞提供受限空间，同时促进细胞的增殖、分化。上述结构揭示钛基骨修复材料表面特性与细胞相互作用机制，为临床构建出生物相容性优异的钛基骨修复材料提供理论依据。