新型多维液相色谱固定相的研制

Monodisperse polyvinylbenzene-divinylbenzene (PSTDVB) particles (5-50 mm) were prepared using one-step seed polymerization. A new and simple method for preparation of latex-coated pellicular cation exchanger was introduced. Monodisperse agglomerated pellicular cation chromatographic stationary phase was prepared for the first time.The monodisperse polyvinylbenzene-divinylbenzene (PSTDVB) particles (10 mm) as substrate beads were functionalized with a tertiary amine on the surface firstly and then attached a layer of sulfonated latex particles (0.2 mm) to give the desired packing. Its chromatographic properties were evaluated by using alkali metals (Li+, Na+ , NH4+, K+) and alkaline earth metals (Mg2+, Ca2+). The results showed that the packing is of good resolution, low detection limit, and excellent duplicate for the common inorganic cations.

研究解决复杂体系样品的快速分离、分析是当代分析化学的发展趋势之一。本研究拟合成不同粒度的单分散高交联的多孔聚合物微球对微球进行化学修饰和在其表面附聚阴阳离子乳胶形成具有分子排阻、阴阳离子交换与反相色谱等多功能的二维、三维和四维色谱固定相。用于生物和环境样品的直接进样快速分分析，促进我国分析化学和生命科学的发展。