基于口腔病菌的两种民族药物质基础及其抑菌机制研究

Oral diseases are common disease and frequently-occurring disease in people’s life...As oral diseases seem to be inoffensive, they can’t lead patients’attention and vigilance,therefore not only other systems of the patients will be affected but also related diseases will be caused. Modern research shows that oral diseases which are related to some serious diseases,such as diabetes,coronary disease,chronic gastritis and so forth,should attract considerable attention.In oral health and prevention,ethnomedicine of Chinese traditional medicine which has advantages in resources and long-term folk medication experience,has become the important thesaurus of developing oral health products and medicine...In 2012 and 2014,two new compounds which were named Rubesanolide D and Fladin A were isolated respectively from different Chinese herbs by the applicants.At the same time,the cooperative research of applicants and Prof. Jeffrey A.Banas from University of Iowa showed that both the compounds can block the formation of oral biofilm and have an obvious antibacterial effect.Based on that,this project has chosen two kinds of ethnic herbs which are commonly used to treat the oral diseases from Buyi and Shui nationalities to study on the effective components and inhibiting activity of oral diseases and search for the active fractions and leading compounds which have healthy functions and therapeutical effect.

口腔疾病是人体的一种常见病和多发病。. 口腔疾病由于没有明显的危害性，未引起患者警觉和重视，进而影响人体其它系统，诱发关联疾病的产生。现代研究表明，口腔疾病与某些重大疾病有一定相关性，如糖尿病、冠心病、慢性胃炎等，应引起人们的关注和重视。在口腔保健防治方面，中药民族药有资源和长期民间用药经验的优势，是开发口腔保健品及防治药物的重要宝库。. 2012和2014年，申请者分别从不同中草药中分离到两个新化合物RubesanolideD和FladinA与美国爱荷华大学JeffreyA.Banas教授合作研究时发现：两个单体能阻断口腔生物膜的形成，有很好的抑菌作用。本项目以此为基础，选择布依族、水族常用于治疗口腔疾病的两种民族药材，进行口腔病菌抑制作用和有效成分的研究，寻找有保健功能或治疗作用的活性部位和先导化合物。

本项目以布依族、水族常用于治疗口腔疾病的两种民族药——水族药哈壳（ Isodon amethystoides (Benth) Hare）和布依族药昂加热（Isodon coesta (Buch.-Ham..ex.D. Don) Hara）为研究对象，开展药效物质基础研究，寻找具有治疗口腔疾病活性物质及先导化合物。项目在实施过程中，严格按照国家基金委对项目的管理办法，严格按照课题合同任务书的研究内容、研究目标、经费预算和考核指标执行。. 本项目较好地完成了两种民族药的化学成分及生物活性研究工作，项目取得成果的总体情况如下：. ① 从两种民族药中分离并鉴定了89个单体化合物，其中发现新骨架1个，新化合物21个。. ② 选取15个单体化合物开展抗菌、抗病毒、抗肿瘤活性筛选，发现5个单体成分具有潜在的抗菌、抗病毒、抗肿瘤活性。. ③ 共发表论文 7 篇，其中 SCI 论文 2 篇，核心期刊论文 4 篇。. ④ 晋升中级职称2人，培养硕士研究生3名。. 综上所述， 本项目较好地完成了合同规定的考核指标，后期将继续寻找先导化合物及相关活性作用机制研究，为新药开发及资源合理利用奠定科学