基于CNP-cGMP信号通路探讨健脾益气法对脾虚证大鼠胃窦平滑肌的干预机制

Basically,according to the previous research,we surpose the genesis of "dysfunction of the spleen in transportation" is related to antrum smooth muscle CNP-NPR-B-pGC-cGMP signal pathway.The project would plan to establish the rat model of"dysfunction of the spleenin transportation"(namely the syndrome of spleen deficiency and gastrointestinal dynamic disfunction)；apply nuclide solid empty method to observe the gastrointestinal evacuation of the deficiency of spleen rat.We would explore the mechanism of "dysfunction of the spleen in transportation" in accordance with the antrum smooth muscle CNP-NPR-B-pGC-cGMP signal pathway by organ bath study、immunohistochemistry、radioimmunoassay and RT-PCR.The project would apply the regulation theory of gastrointestinal motion contraction to research the splenasthenic syndrome, to explain the mechanism of "dysfunction of the spleen in transportation" from a totally new point of view, and would open up a new thinking and method for the contemporary research for the spleen and stomach doctrine of Traditional Chinese Medicine.

在前期研究的基础上，我们提出"脾虚失运"的发生与胃窦平滑肌CNP-NPR-B-pGC-cGMP信号转导通路有关。本项目拟采用耗气破气加饥饱失常法制作"脾虚失运"SD大鼠模型，应用核素固体胃肠排空法观察"脾虚失运"大鼠胃肠排空情况。利用离体肌条实验、免疫组织化学、放射免疫分析、RT-PCR等技术，从胃窦平滑肌CNP-NPR-B-pGC-cGMP信号转导通路探讨"脾虚失运"的发生机制。本项目将胃肠运动收缩调控理论运用于脾虚证的研究，以全新的角度阐明"脾虚失运"证的发生机制，为中医脾胃研究开辟新的思路和方法。