Ehrlich反应诱导表面增强共振拉曼散射及其在吲哚类植物生长素选择性检测中的应用

Auxins are the most important phytohormones, which play a vital role in regulating growth and development processes. However, auxin’s content in plant is very low and the detection is easily interfered by other compounds in cell. Therefore, there is an urgent demand for highly sensitive and selective detection technique of auxins from plant in the agricultural production and Chinese crude drug cultivation. In this study, to aim directly at the problems (non-selectivity, low sensitivity and sophisticated sample pretreatment) existed in common analysis of auxins from plant, we will combine high selectivity of Ehrlich-reaction and high sensitivity of surface-enhanced Raman scattering (SERS) spectroscopy analysis, to prepare high SERS active substrates, orientedly synthesize surface-enhanced resonance Raman scattering (SERRS) probe, explore the principle of Ehrlich-reaction-induced resonance Raman scattering and mechanism of Raman signal enhancement after the substrate absorbed target analytes, establish technology platforms for analysis of trace indole-auxins from plant, and realize highly selective identification and ultrasensitive detection for the typical indole-auxins from plant. The anticipate results of this project will extend Ehrlich-reaction-induced SERRS application on the SERS spectroscopy analysis area, open a new pathway to selectively identify and detect the indole-auxins from plant. Meanwhile, it is very important for our country to study the mechanism of molecular action of phytohormones.

生长素是一种重要的植物激素，在植物生长发育过程中起重要的调控作用(且依赖其浓度)，但因其含量极低，加之细胞中其它成分的干扰。因此，发展灵敏和专一的生长素检测技术，在农业生产或中药材栽培等方面具有紧迫需求。本项目拟对传统方法在生长素测定中存在的问题(选择性差、灵敏度低和样品前处理复杂等)，将Ehrlich反应的选择性与表面增强拉曼散射(SERS)光谱的敏感性结合起来，设计制备高SERS活性衬底；定向合成表面增强共振拉曼散射探针；探索Ehrlich反应诱导的共振拉曼散射原理与拉曼信号放大输出机制；建立植物中痕量吲哚类生长素分析技术平台；实现对植物中典型吲哚类生长素的高选择性鉴定与超灵敏检测。本项目预期成果将会极大扩展Ehrlich反应诱导表面增强共振拉曼散射在SERS光谱分析领域中的应用，从技术层面上为植物中吲哚类生长素检测提供新的途径，同时对深入研究植物激素分子作用机理具有十分重要的意义。

生长素是一种重要的植物激素，在植物生长发育过程中起重要的调控作用(且依赖其浓度)，但因其含量极低，加之细胞中其它成分的干扰。因此，发展灵敏和专一的生长素检测技术，在农业生产或中药材栽培等方面具有紧迫需求。本项目针对传统方法在生长素测定中存在的问题(选择性差、灵敏度低和样品前处理复杂等)，将Ehrlich反应的选择性与表面增强拉曼散射(SERS)光谱的敏感性结合起来，(1)设计制备出3种高SERS活性衬底；(2)定向合成了表面增强共振拉曼散射探针(IBA-PDAB)；(3)探索了Ehrlich反应诱导的共振拉曼散射原理与拉曼信号放大输出机制；(4)建立了植物中痕量吲哚类生长素分析技术平台，实现了对植物中典型吲哚类生长素的高选择性鉴定与超灵敏检测(2.0 nM)。本项目发表论文14篇、获得授权发明专利1项、培养硕士研究生毕业10名和博士研究生毕业1名，其主要成果将会极大扩展Ehrlich反应诱导表面增强共振拉曼散射在SERS光谱分析领域中的应用，从技术层面上为植物中吲哚类生长素检测提供新的途径，同时对深入研究植物激素分子作用机理具有十分重要的意义。