耐铊微生物菌株对Tl(I)/Tl(III)形态转化机制研究

Thallium (Tl) is a severe toxicity heavy metal, which has two chemical speciatons such as Tl(I) and Tl(III). However, the toxicity varies greatly from each speciation and some researchers found that they could transform from each other by the specific microbes. However, little is known on the mechnissm of Tl(I) /Tl(III) transformation. It’s of significant importance to affect the specaiton transformation by means of possible ways including microbiological way. In this proposal, the effect of Tl-tolerant strains on the the specaiton transformation will be studied based on the previous work. The concentrations of Tl(I) and Tl(III) in the liquid substrate added Tl-tolerant strains will be characterized by microcolumn separation with ICP-MS. Besides, the kinetics of Tl(I)/Tl(III) will also be investigated under the different conditions. By using XANES technology, the concentration variations of Tl(I) and Tl(III) in the sub-cellular organelles will be examined. Moreover, the different expression genes will indicate that some kind of metabolisms, even the proteins and enzyme, may be employed to promote the redox activities based on the transcriptome and bioinformatics analysis. Through all these results, the underlying mechanism of Tl-tolerant strains on the Tl speciaton transformation will be revealed. This will shed lights on the the micro-remediation Tl-contaminated soil.

铊是典型毒害重金属元素，在自然界有两种形态：Tl(I)和Tl(III)，两者生物毒性存在巨大差异，有研究表明两者在微生物作用下可发生转化，但相关作用机理研究较少。在前期工作基础上，本项目以耐铊菌株作用下Tl(I)/Tl(III)的形态转化特征及机制为研究目标，a) 利用微柱分离法和ICP-MS查明培养介质中Tl(I)/Tl(III)含量变化特征，b) 构建氧化还原动力学曲线，考察主控因子对Tl(I)/Tl(III)组成变化特征的影响，c) 应用XANES技术分析细胞内Tl(I)/Tl(III)的分布特征，d) 基于转录组技术阐明影响形态铊转化过程的相关功能蛋白（酶），应用生物信息学解析其影响机制。通过本项目研究，将组建对Tl(I)/Tl(III)具有氧化或还原性的功能性菌群，获取一批丰富的生物地球化学数据，将丰富铊的表生生物地球化学循环的认识，还将为铊污染地区的污染防治等提供科学依据。

铊是典型毒害重金属元素，在自然界有两种形态：Tl(I)和Tl(III)，两者生物毒性存在巨大差异，有研究表明两者在微生物作用下可发生转化，但相关作用机理研究较少。在前期工作基础上，本项目以耐铊菌株作用下Tl(I)/Tl(III)的形态转化特征及机制为研究目标，a) 利用微柱分离法和ICP-MS查明培养介质中Tl(I)/Tl(III)含量变化特征，b) 构建氧化还原动力学曲线，考察主控因子对Tl(I)/Tl(III)组成变化特征的影响，c) 基于转录组技术阐明影响形态铊转化过程的相关功能蛋白（酶），应用生物信息学解析其影响机制。通过本项目研究，将组建对Tl(I)/Tl(III)具有氧化或还原性的功能性菌群，获取一批丰富的生物地球化学数据，将丰富铊的表生生物地球化学循环的认识，还将为铊污染地区的污染防治等提供科学依据.。