稀土镧和铈对东北大豆籽粒化学品质形成的影响及其调控机制

Revealing the physiological mechanism and control principle that rare earth is advantageous to the formation of soybeans' chemical quality improves the efficient utilization of soybean quality resources and optimizes the soybean products quality and guides the tuning soybean cultivation and breeding. This has important theoretical significance and wide application prospect. Rare earth agricultural has a long history .It indeed improve the yield and quality of agricultural and sideline products, but the regulation mechanism is not yet clear; The strong impact of foreign genetically modified soybeans makes Chinese non-gom soybeans industry face an unprecedented survival crisis. Based on this,three different quality characters of northeast soybeans were used as experimental materials.Synthetically researching the impacts of lanthanum, cerium and their interaction on soybean chemical quality, photosynthetic characteristics, carbon and nitrogen assimilate translocation and related enzyme activity . Investigate the regularity that the impact of the Lanthanum and cerium on soybean quality. Make the mechanism that rare earth controls the quality of soybean clear. Chemical analysis of the United Nations Food and Agriculture Organization /World Health Organization (FAO/WHO) model and whole egg model are used. Systematically evaluate the nutritional value of soybean processed by different rare earth. Using the differential proteomics method explores the mechanism that rare earth regulates the quality of soybeans, to lay a foundation for further improvement of soybean qualities. Then provide technical support for the development of our country's non-gmo soybean industry and provide a scientific basis in the development of rare earth applied to high quality cultivation techniques of soybeans.

揭示稀土对大豆籽粒化学品质形成的生理机制及调控原理，对深化优质大豆资源高效利用和优化大豆产品质量、指导大豆调优栽培和育种均具有重要理论意义和广泛应用前景。稀土农用历史悠久，且确实能提高农副产品的产量和品质，但其调控机理尚未明确；国外转基因大豆的强烈冲击使中国本土非转基因大豆产业面临空前的生存危机。基于此开展以3个不同品质特征的东北大豆为试验材料，综合研究镧、铈及其交互作用对大豆化学品质形成、光合特性、C/N物质运转和相关酶活性的影响，探讨镧、铈对大豆品质的影响规律，明确稀土调控大豆品质的机制。采用化学分析法中的联合国粮农组织/世界卫生组织(FAO/WHO)模式和全蛋模式，系统评价不同稀土处理大豆的籽粒营养价值，并利用差异蛋白质组学方法探究稀土调控大豆品质的机理，旨在为进一步改良大豆品质奠定基础，进而为振兴我国非转基因大豆产业提供技术支撑，为拓展稀土在大豆优质栽培技术中的应用提供科学依据。

本项目以东北典型的大豆高蛋白的东农42，高脂肪的东农47和兼用型的东农52为试验材料，在大豆苗期和初花期分别进行叶面喷施，探讨稀土镧、铈对成熟期大豆籽粒化学品质形成的生理机制及调控原理。研究表明，叶面喷施适宜浓度的稀土镧、铈溶液可以显著地提高不同品质型大豆的蛋白质含量、脂肪含量、必需氨基酸的比例、氨基酸评分、氨基酸比值系数分、化学评分、11S/7S蛋白亚基的比值，并可以调控产生差异蛋白，达到优化大豆品质，提高大豆营养价值的目的。本研究为进一步改良大豆品质奠定基础，进而为振兴我国非转基因大豆产业提供技术支撑，为拓展稀土在大豆优质栽培技术中的应用提供科学依据；对深化优质大豆资源高效利用和优化大豆产品质量、指导大豆调优栽培和育种均具有重要的理论意义和广泛的应用前景。本研究按照项目研究计划开展工作，完成了计划中所有研究任务，达到了研究目标，取得了良好的研究成果。本研究已经发表论文6篇，会议论文摘要9篇，待发论文6-8篇 ，参加国内会议2次；指导的学生获得国家级奖1项；培养硕士研究生11人，其中毕业7人。