单颗粒金属纳米结构的高通量宽场成像光谱表征技术研究

Single-particle localized surface plasmon resonance (SP-LSPR) scattering spectral characterization is an important analytical technology for studying physical and chemical properties of metallic nanostructures at their surfaces/interfaces and SP-LSPR biochemical sensing realization. However, present dominant method of using dark-field microscopy and imaging spectrograph in conjunction is a type of serial characterization method, which has very limited power in acquiring spatial and spectral information and very low detection throughput. On the basis of previous research, the project plans to adopt the principle of imaging Fourier transform spectroscopy and combines it with dark-field microscopic illumination to develop a wide-field imaging spectroscopy technology for improving the afoementioned defficiencies of traditional characterization method. Through the study of instrumentation setup and performance testing, a dark-field imaging Fourier spectrometer prototype that can obtain spectrum at every pixle from a wide filed is expected to set up. Through the application experiment research, the feasibility of applying this prototye for SP-LSPR scattering spectral characterization is to be explored and demonstrated. Finally, the methodology that based on this high-resolution dark-field imaging Fourier spectroscopy for high-throughput metallic nanostructures' SP-LSPR scattering spectral characrterization is expected to establish.This study may not only provide a new physical and chemical characterization technology at single-particle surface/interface for the basic research of nanoscience, it is also of significant value for the realization of high-tech applications such as biosensing, drug screening and environmental monitoring that based on spectral analysis of single micro-/nano-structures.

单颗粒局域表面等离子体共振(SP-LSPR)散射谱表征是研究金属纳米结构表面/界面物理化学性质和实现SP-LSPR生化传感的重要分析技术。然而目前主流的暗场显微摄谱法是一种串行表征方法，其空间和光谱信息同时获取能力及检测通量均十分有限。本课题在前期工作的基础上，拟采用成像傅里叶变换光谱技术原理，结合暗场显微照明，发展一种宽场成像光谱测量技术，以提升传统表征方法的不足。通过仪器研制和测试，实现一部可同时测得宽视场中每一像素处光谱的暗场成像傅里叶光谱仪原型机；通过应用实验研究，探索和展示该原型机应用于高通量SP-LSPR散射谱表征的可行性；最终建立基于暗场成像傅里叶光谱技术的高通量金属纳米结构SP-LSPR散射谱表征方法。本课题的实施可为纳米科学的基础研究提供一种新型的单颗粒表面/界面物理化学表征技术，对基于单个微纳结构光谱分析的生物传感、药物筛选以及环境监测等高技术应用实现亦具有重要的价值。

单颗粒局域表面等离子体共振(SP-LSPR)散射谱的光学表征是研究金属纳米结构表面/界面物理化学性质和实现SP-LSPR生化传感的重要分析技术。然而目前主流的暗场显微摄谱法是一种串行表征方法，其空间和光谱信息同时获取能力及检测通量均十分有限。本项目采用成像傅里叶变换光谱技术原理，结合暗场显微照明，发展了一种宽场成像光谱测量技术，可以在一次测量中获取宽视场里每一个像素处的光谱；通过对光谱表征时空间复用率的提升增加光谱表征的通量，从而大幅改善传统表征方法的不足。通过仪器研制和测试，成功搭建了一套暗场成像光谱仪原型机系统。该原型机的工作光谱范围覆盖400-1000纳米、最高光谱分辨率优于0.5纳米、最大成像大小550万像素、最高空间分辨率可优于0.5微米。原型机系统还具有图形化的仪器控制和数据处理软件，可以良好的自动化程度进行仪器控制、数据采集和数据处理。通过对金属纳米颗粒以及光子晶体微球等多种微纳颗粒材料的表证演示实验，初步研究了适合该方法测量的微纳颗粒材料种类、大小和制样方法；探索了满足SP-LSPR散射谱测量中光谱、时空和灵敏度性能之间制衡关系最优化条件下的原型机仪器工作参数，展示了该方法应用于高通量SP-LSPR散射谱表征的可行性和优势；最终建立了基于暗场成像傅里叶光谱技术的高通量金属纳米结构SP-LSPR散射谱表征方法。本课题的实施可为纳米科学的基础研究提供一种新型的单颗粒表面/界面物理化学表征技术，对基于单个微纳结构光谱分析的生物传感、药物筛选以及环境监测等高技术应用实现亦具有重要的价值。