家蝇对拟除虫菊酯抗性及其Na+通道结构功能的研究

The resistance level and mechanism of housefly, selected in the laboratory by deltamethrin and permethrin, was studied by bioassay and biochemical methods. The difference of cDNA encoding the Na+ channel between insecticide- resistant and susceptible housefly strains was investigated by PCR-based methods. Results shown that deltamethrin resistance was associated with the decrease of affinity of Na+ channel, while permethrin resistance was not.

通过Na+通道不同受体的专一性抑制剂河豚毒素乌头碱和蝎子毒素等及溴氯菊酯、氯菊酯对獬婢挣タ剐院兔舾衅废导矣惺嗌窬头诺挠跋欤芯坎煌嘈湍獬婢挣ピ贜a+通道上的作用位点。探索聚合酶链式反应（PCR），Na+通道基因可隆等生物分子工程技术。以进一步了解Na+通道结构功能的变异与Kdr抗性的关系，为合理使用杀虫剂提供理论依据。