仿生多肽自组装纳米探针的高特异性肿瘤成像新方法研究

Malignant tumor (cancer) have become a serious threat to human health and life, it is significantly important for early diagnosis and the improvement of its cure by means of developing highly sensitive and highly specific tumor imaging. Based on the principle of self-assembly of amphiphilic polypeptide, this project proposes a new method for the synthesis of controllable biomimetic peptide self-assembled nanoprobe; Furthermore, in combination with the molecular recognition mechanism of peptides, we will also explore the adjustable method for the specificity response of the multifunctional polypeptide nanoprobe. It was expected to provide a simple and practical biomimetic polypeptide self-assembly nanoprobe for high sensitive and specific tumor imaging with good controllability, biocompatibility and responsiveness. Briefly, the research findings of the project not only have high academic value, but also hold great bioapplication prospect in polypeptide self-assemblies and the corresponding technology development.

恶性肿瘤（癌症）严重着威胁人类的健康和生命，高灵敏高特异性的肿瘤成像对于肿瘤的早期诊断和指导治疗具有非常重要的意义。本项目拟结合多肽自组装技术，凝练基于两亲性多肽分子自发组装一般构建原则，发展可控的仿生多肽自组装纳米探针的合成新方法；进一步结合多肽的分子识别机理，探讨功能化多肽纳米探针的特异性响应调控方法，以期为可控、稳定、生物相容性良好的多功能多肽纳米探针用于肿瘤的高灵敏高特异性成像提供简单实用的新技术平台。总体上，项目的研究成果不仅具有较高的学术价值，也将在多肽自组装的相关研究以及相应的技术开发方面具有极大的应用前景。

开发新型的多功能纳米诊疗探针，实现恶性肿瘤的有效诊断与治疗具有重要意义。针对当前肿瘤诊断与治疗所面临的靶向识别、早期诊断和特异性治疗等问题与挑战，利用功能化纳米材料和DNA自组装技术，设计和构建了一系列新型纳米探针，从细胞水平到活体层面系统开展灵敏、特异、原位、实时的肿瘤成像诊断，揭示其发生发展过程中的规律和机制，并进一步用于指导治疗。相关研究对提高患者治愈率和生存率具有十分重要的意义。在项目的资助下，相关研究成果已经在Chemical Science (1篇)，Analytical Chemistry (5篇)，Chemical Communications (3篇)，Biosensors and Bioelectronics (1篇)，Nanoscale (1篇)，ACS Sensors (1篇)等重要国际学术杂志上发表15篇，其影响因子均大于4.0，均标注本项目资助。同时，项目组培养以及协助培养博士研究生6名，硕士研究生4名。