太湖沉积物中典型抗生素与重金属的耦合作用及其环境行为

The surface sediment is an important carrier for migration and transformation, and a main destination for antibiotics and heavy metals in the water. The coupling interaction between antibiotics and heavy metals not only affects their migration, transformation, destination,but also the biological activity and ecological risk of antibiotics and heavy metals in the environment. In this project, OECD batch adsorption method is employed to reveal the coupling mechanism and environmental behaviors of typical antibiotics and heavy metals (Tetracycline, lead and cadmium) in sediments. Different areas which are influenced by human activities, such as Lakeside zone, estuary, lake bay and drinking water sources are selected in Taihu lake. Special and temporal distribution characters of antibiotics and heavy metals in sediments of Taihu Lake are analysised. The coupling mechanism of antibiotics and heavy metals is researched by using the capillary electrophoresis, ICP-MS,HPLC,UV and fluorescence spectroscopy. Combined with computational chemistry software of Gaussian 09 and Materials Studio 5.0, the environmental behaviors and its coupling mechanism of antibiotics and heavy metals are simulated and analyzed, their mutual relationship and process of antibiotics and heavy metals are explored from the molecular perspective. The ecological toxic effects of antibiotics and heavy metals are assessed according to the toxicology methods and modern molecular biology techniques. Results of this project may enhance our understanding for the environmental behaviors and coupling mechanism of antibiotics and heavy metals, which can provide technical supporting and theoretical basis for assessing the ecological effects and environmental risk of antibiotics and heavy metals combined pollution in Taihu lake.

表层沉积物是水体中抗生素和重金属迁移转化的重要载体，也是其在水体中的主要归宿。抗生素与重金属的耦合作用，不仅影响二者在环境中的迁移、转化和归宿，同时也改变其生物活性和生态风险。本项目以太湖典型抗生素与重金属为研究对象，选取湖滨带、入湖河口、湖湾、饮用水源地等不同区域，分析抗生素与重金属在太湖沉积物中的时空分布特征；采用OECD吸附-解吸批量平衡法，借助毛细管电泳、ICP-MS、HPLC、紫外、荧光光谱等分析手段，探讨沉积物中抗生素与重金属的耦合过程机制与环境行为；应用计算化学软件，通过实验研究和理论模拟，从分子水平上进一步揭示抗生素与重金属的耦合作用过程机制；利用毒理学方法与现代分子生物学技术，评估抗生素和重金属的生态毒性效应。研究成果将有助于明晰沉积物中抗生素与重金属的耦合作用、耦合机制及其耦合过程，为正确认识和评价抗生素和重金属复合污染引起的生态效应及其环境风险提供技术支撑和理论依据。

表层沉积物是水体中抗生素和重金属迁移转化的重要载体，也是其在水体中的主要归宿。抗生素与重金属的耦合作用，不仅影响二者在环境中的迁移、转化和归宿，同时也改变其生物活性和生态风险。本项目以太湖典型抗生素与重金属为研究对象，首先优化了水环境中特征污染物的吸附富集方法和金属离子的荧光检测方法，调查了不同区域点位抗生素与重金属在太湖水环境中的赋存特征；通过实验研究和理论模拟，从分子水平上进一步揭示抗生素与重金属的耦合作用过程机制；以沉积物中氧化铝和粘土为研究对象，重点研究了抗生素和重金属的吸附、光解等环境行为，阐明了光照和重金属作用下抗生素的降解特性；以发光菌为研究对象，较为系统的开展了抗生素和重金属的环境毒性及其效应，为正确认识和评价抗生素和重金属复合污染引起的生态效应及其环境风险提供技术支撑和理论依据。