泡腾作用辅助离子液体分散液相微萃取技术在蜂蜜中有机污染物残留分析的应用研究

This project aims to establish the effervescence-assisted ionic liquids based Dispersive liquid-phase microextraction and using it as new sample pretreatment method for determination organic pollutants' residues in honey samples. With this method, the typical organic pollutants such as antibiotics and pesticides will be selectively extracted with Ionic liquids based liquid-phase micro-extraction method. With a variety of analytical instruments, the main factors which affect the efficiency of the extraction process will be studied during the research process. In addition, the extraction conditions will be optimized to increase the extraction efficiency and reduce the time of extraction. Finally, simple and rapid ionic liquids based liquid-phase microextraction method will be established, which has the characteristics of high recovery rate, low detection limit, high sensitivity, matrix interference resistance. The method will be used to effectively analysis the multicomponent organic pollutants in honey samples. The purpose of this research is to provide not only high sensitivity and high efficient sample preparation technology for the analysis organic pollutants in honey samples, but also theoretical foundation and guiding principle for the establishment of the technical standard for the maximum organic pollutants'residue limit in honey. The proposed method will integrates extraction and preconcentration in one step. It is a new, simple, rapid and environmental friendly sample pretreatment method for trace analysis of organic pollutants. This research has significant implications for expanding the application area of new and environmental friendly sample pretreatment technology in the field of organic pollutants residue analysis science. This project can improve the quality and safety of honey in our country, and it has important academic significance and practical application value.

本项目旨在建立适用于蜂蜜中有机污染物残留检测分析的泡腾作用辅助离子液体液相微萃取样品前处理技术，考察影响该技术对蜂蜜样品中抗生素和农药等典型有机污染物萃取效率的主要因素，优化萃取条件，提高该方法的萃取效率和抗基质干扰能力，并通过与色谱分析手段结合的方式，评估其应用于实际蜂蜜样品中有机污染物检测工作的可行性，建立蜂蜜中痕量及超痕量有机污染物的多组分快速分析方法。该技术是一种集萃取浓缩为一体、简便快速、环境友好的新型样品前处理技术。开展本项目的研究将为蜂蜜样品中有机污染物检测提供灵敏度高、高效的样品前处理技术，为将来蜂蜜中有机物残留分析标准的制定和修订提供理论基础和科学依据，拓展新型环境友好样品前处理技术在有机污染物残留分析领域的应用研究。该项目的完成对于提高我国蜂蜜产品质量安全水平有良好的促进作用，具有有重要的学术和应用价值。

本研究建立了一系列用于蜂蜜、果汁、茶及环境水样中农药残留分析的泡腾作用辅助离子液体微萃取样品前处理技术，并将该技术与高效液相色谱联用，是一种简便快速、环境友好的适用于测定食品、环境样品中有机污染物残留的新型样品前处理技术。研究围绕泡腾辅助样品萃取这一核心问题，在分散液相微萃取方面开展了泡腾辅助离子液体、泡腾辅助离子液体悬浮固化，泡腾辅助TiO2纳米流体几方面的工作；在分散固相微萃取方面开展了泡腾辅助离子液体涂覆的磁性环糊精/坡缕石、泡腾辅助注射器中坡缕石负载β-环糊精、超支化有机硅材料泡腾辅助固相微萃取几方面的工作。采用相关的泡腾辅助技术检测了蜂蜜等样品中常见的杀虫剂、杀菌剂及杀螨剂残留。 .研究结果表明：泡腾辅助样品萃取方法具有快速、回收率高、富基因子高、易操作、设备简单、材料低耗、环境友好等优势。通过泡腾分散显著加快了萃取过程，避免了有机分散剂的使用。将绿色溶剂离子液体代替普通有机溶剂用于样品前处理技术中，拓展了该方法的应用范围，同时还减少了由于分析过程中使用挥发性有机溶剂对人类健康和环境的危害。该技术在实际样品中取得的较高的萃取回收率，证实该方法在检测复杂基质中的有机污染物残留方面具有巨大的应用潜力。其中泡腾片的制作是较为耗时的一步，可以通过提前准备，于干燥环境中储存运输来克服此缺点，萃取过程在现场取样及野外采样检测方面具有潜在的应用价值。.泡腾作用辅助离子液体微萃取样品前处理技术是一种简单、快速、绿色、高效的方法，可用于检测蜂蜜等复杂机制样品中有机污染物残留。该方法的建立推进了泡腾作用及离子液体在绿色环保分析领域的应用进程 ，对于有机污染物分析研究有重要研究价值，有助于促进绿色化学、环境分析、食品化学和物理化学等分支学科的进一步交叉融合。