测定PM2.5中典型溴代阻燃剂的实时荧光免疫PCR生物条形码分析新方法研究

PM2.5 has been firstly listed into the "Ambient air quality standard" (GB3096-2012) as a type of general pollution. As we know, PM2.5 is the primary pollution factor to form haze, and could be harm to human health. Specially, brominated flame retardants (BFRs) could be absorbed onto the surface of PM2.5 and migrate further. The interaction between BFRs and PM2.5 will amplify the hazard to human health. The amount of BFRs on PM2.5 is relative low. Therefore, for determination, the extractive enrichment- chromatography- mass spectrum technology is common used. However, these methods have some disadvantages, such as complex pre-treament steps and requiring expensive instrument, which make them unfit for on-the site analysis and pollution screening. So, establishing a kind of new, rapid, simple, and high throughput analysis and detection method is very important in natural research plan. Bio-barcode is a new type of analytical technology basis on the principle of molecular biology. In the past 10-years, this technology has acquired great development. However, the researches using bio-barcode technology for detecting persistent organic pollutants on PM2.5 are not reported. So, this project will carry out the relative researches to establish some new high throughput analytical methods in the principle of bio-barcode technology and apply to detect BFRs on PM2.5. We think these researches will fill up the blank in field of analysis and detection, and have important scientific and practical significance. The research achievements will include 3 patents of invention and 7 SCI papers, with independent intellectual property rights.

我国新的《空气环境质量标准》（GB3096-2012），首次将PM2.5纳入空气污染物一般项目，它是形成雾霾的主要污染因子，是危害人体健康的杀手。溴代阻燃剂被PM2.5吸附后远程传输，并与PM2.5相互作用对环境和人体健康造成更大的危害，在PM2.5中的其含量很低，通常使用萃取富集-色谱-质谱联用技术进行定量测定，前处理复杂和使用的仪器昂贵，不适合现场分析和污染源的普查；建立快速简便高通量的分析监测新方法是国家科技计划中的任务之一。生物条形码是一种新的分子生物分析技术，该技术发展10年来，还未见文献报道过测定PM2.5中持久性有机污染物的生物条形码分析方法的研究；本项目拟率先开展测定PM2.5中典型溴代阻燃剂的高通量生物条形码分析新方法研究，将填补持久性有机污染物分析监测研究领域中的空白，具有重要的科学意义和应用价值；研究成果具有自主知识产权，将获3项发明专利，发表SCI论文7篇。

在PM2.5中的溴代阻燃剂含量很低，通常使用萃取富集-色谱-质谱联用技术进行定量测定，前处理复杂和使用的仪器昂贵，不适合现场分析和污染源的普查；建立快速简便高通量的分析监测新方法是国家科技计划中的任务之一。本项目在国家基金的资助下, 率先开展了测定PM2.5中典型溴代阻燃剂的高通量生物条形码分析新方法研究，填补持久性有机污染物分析监测研究领域中的某些空白，研究成果具有自主知识产权，申请了6项发明专利，已在国外SCI收录期刊发表论文16篇。. 1. 合成了典型溴代阻燃剂TBBPA、HBCD和TBC的半抗原、全抗原和抗体的设计、制备和纯化；在SCI收录刊物发表相关的研究论文2篇，申请发明专利2项；. 2. 设计和制备了金纳米探针、碳量子点探针、条形码DNA的；研究了抗体效价及特异性与溴代阻燃剂单体、溴代阻燃剂半抗原和溴代阻燃剂全抗原分子结构之间的构效理论研究，建立QSAR模型；在SCI收录刊物发表相关论文3篇，申请发明专利2项。 . 3. 研究免疫PCR生物条形码分析新方法和实时荧光定量免疫PCR生物条形码分析新方法；在SCI收录刊物发表论文8篇，申请发明专利2项。 . 4.使用新方法，测定了上海市化工区和城区PM2.5中典型溴代阻燃剂TBBPA、HBCD和TBC的含量，并与国标方法比较新方法的可靠性和优缺点，在SCI收录刊物发表论文3篇。. 本项目的完成在研究测定环境中溴代阻燃剂的实时荧光免疫PCR生物条形码检测理论与技术方面有了新的突破，并为其它持久性有机污染物的识别、筛选和分析监测提供新思路，奠定了实时荧光免疫PCR生物条形码分析理论基础，并提供技术支撑。