饮用水典型消毒工艺中微囊藻毒素MCLR消毒副产物生成机制及其生物毒性效应评价与调控研究

Evaluation and regulation on the generative mechanism and biological toxicity of microcystin disinfection by-products is an important guarantee of drinking water safety, and has great significance to prevent and control microcystin induced biological risk. In this study, the typical microcystin MCLR is selected as the target of disinfection, and the generative mechanism of MCLR disinfection by-products in typical processes is analyzed by LC/MS and MS/MS. After chromatography preparation, the novel evaluation method for the biological toxicity of MCLR disinfection by-products is established by combining molecular/cell toxicity experiments and molecular docking simulation. Finally, based on the generative mechanism of MCLR disinfection by-products and their biological toxicity features, the related disinfection processes are optimized or adjust to remove MCLR effectively and reduce the risk of its disinfection by-products. This study not only contributes to a comprehensive cognition on the hazards and control strategy for microcystin disinfection by-products, but also offers valid technique support for the safety of drinking water supply.

微囊藻毒素消毒副产物生成机制及其生物毒性效应的评价与调控是饮用水安全供给的重要保障，并对全面认识和防治微囊藻毒素诱发的环境风险具有重要意义。本研究拟以我国水华污染中常见的微囊藻毒素MCLR作为该类毒素消毒调控的契入点,利用液相色谱-质谱、质谱-质谱联用技术建立多种典型消毒工艺中MCLR消毒副产物生成机制的分析方法；然后在色谱制备的基础上结合蛋白磷酸酶/肝细胞毒性实验与分子对接模拟，建立MCLR消毒副产物生物毒性效应评价的新方法，进一步完善MCLR及其消毒副产物环境风险评估；最后依据MCLR消毒副产物的生成机制及其生物毒性效应特征，探讨通过饮用水消毒工艺的优化与调整，调控MCLR消毒副产物二次污染的可行性。本研究有利于全面认识微囊藻毒素及其消毒副产物的危害与调控策略，并能够为饮用水的安全供给提供科学的技术支持与保障。预计申请专利1-2项，发表SCI论文5-7篇，其中IF≥3的3-4篇。

本研究涉及饮用水典型消毒工艺中微囊藻毒素消毒副产物生成机制及其生物毒性效应评价与调控的研究。本研究结合LC分离与MS、MS/MS鉴定技术，建立MCLR、MCRR消毒副产物结构准确解析的新方法，研究了不同消毒工艺条件下MC消毒副产物的类型特征、生成机制及相应的丰度变化；结合蛋白磷酸酶活性与分子对接理论模拟，从分子水平上阐明MCs消毒副产物抑制蛋白磷酸酶活性的发生机制，建立了评价MC消毒副产物生物毒性效应的新方法；在MC消毒副产物生成机制和生物毒性效应分析的基础上，依据MCLR消毒副产物类型分布和浓度水平等指标，优化并调整饮用水消毒工艺条件，完善MCLR及其消毒副产物生物风险的调控技术。目前已发表SCI论文6篇，其中IF≥3的5篇。