自制口服DNA疫苗的粘膜表达部位与诱导肠IEL效应机制的研究
基本信息
结项摘要
1.构建可在真核细胞内表达Ag85A基因的质粒，以减毒鼠伤寒沙门氏菌苗为运载体，制成可供口服的DNA疫苗，经口途径投与小鼠。2.观察该疫苗对小鼠肠道IEL及其亚群生物学活性的影响, 进行口服DNA疫苗的粘膜局部免疫应答效应的研究，并对其作用机理加以探讨。3．检测Ag85A基因的表达产物在肠粘膜的分布，通过在抗原提呈细胞，肠上皮细胞或M细胞中的定位，阐明口服DNA疫苗诱导肠道局部粘膜免疫应答的机制，
项目摘要
项目成果
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数据更新时间：2023-05-31
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