对辣椒炭疽病菌具拮抗活性的光合细菌蛋白纯化及其作用机理研究

Pepper Anthraenose caused by Colletotrichum spp. is one kind of the most economically destructive diseases of pepper throughout the world. As the climate is suitable,The yield loss was the heaviest (30%-50%). The current control included breeding for breeding for disea-se resistance, agricultural control and chemical control, but the effect is unfavorable. So the biological control has become an important way to this disease for its safe, efficient and pollution-free features, which was attentioned by people. The rhodopseudomonas palustris strain PSB07-16 was isolated and screened the strong antagonistic bacteria against the Colletotrichum gloeosporioides Pena. from photosy- nthetic Bacteria Strains-Library by confrontation test. In this study, the antifungal proteins produced by PSB 07-16 was primary isolated and purified ， and the possible antiftmgal mechanism was studied．The antifungal proteins be of value to the biological pesticide.

辣椒炭疽病(Pepper Anthracnose)是辣椒的一种重要病害。如气候适宜，可导致辣椒减产30%-50%。当前对辣椒炭疽病的防治主要通过选育抗性品种等农业防治和化学防治来控制，但因效果不理想，或易引发病原菌抗药性、环境污染等问题，人们更多的将目光投向安全高效的无公害生物防治。针对这一现状，本研究利用本课题组生防菌菌种库，以湖南辣椒炭疽病优势病原菌胶胞炭疽菌为靶标菌，筛选出一株对辣椒炭疽病菌具拮抗作用的光合细菌生防菌株PSB07-16。本项目拟利用层析技术纯化该菌种产生的抗菌蛋白；并解析抗菌蛋白对辣椒炭疽病菌的作用机理，可获得具有自主知识产权的抗菌蛋白质资源。本研究对探索该菌株的生防应用价值、对以抗菌蛋白质为核心的生物农药研究和挖掘都具有重要意义。

辣椒炭疽病(Pepper Anthracnose)是辣椒的一种重要病害。如气候适宜，可导致辣椒减产30%-50%。当前对辣椒炭疽病的防治主要通过选育抗性品种等农业防治和化学防治来控制，但因效果不理想，或易引发病原菌抗药性、环境污染等问题，人们更多的将目光投向安全高效的无公害生物防治。针对这一现状，本研究利用本课题组生防菌菌种库，以湖南辣椒炭疽病优势病原菌胶胞炭疽菌为靶标菌，筛选出一株对辣椒炭疽病菌具拮抗作用的光合细菌生防菌株PSB07-16。本项目拟利用层析技术纯化分离出可能对辣椒炭疽具有活性的蛋白5个，另一活性峰的分离还在进行中。同时针对分离鉴定的5个蛋白进行原核表达载体的构建，从而分离纯化出单一蛋白。而且就总抗菌蛋白对辣椒炭疽病菌的作用机理做了研究。相关研究申请专利共6个。本研究对探索该菌株的生防应用价值、对以抗菌蛋白质为核心的生物农药研究和挖掘都具有重要意义。