高选择性SERS动态探测腺苷类针刺效应物质

The material-based hypothesis for effects of acupuncture in the field of Chinese medicine has aroused great attention in the scientific community, however, the understanding of releasing and transformation of acupuncture substance is still nonplussed, which greatly limits the development of acupuncture medicine. In this research project, for the first time, dynamic SERS method would be used to monitor adenosine release and transformation of acupuncture effect substance. Using the magnetron sputtering nanotechnology, development of synthetic method and principle of core-shell type SERS substrate containing internal standard signal molecules, and functional design on the surface, multifunctional SERS probe owning acupuncture, sampling and enhancement of signal are produced based on the acupuncture needle. The quantitative and selective dynamic SERS detection of the adenosine target molecule is carried out on the model replication of animal osteoarthritis of knee of rats. The relationship of SERS optical signal detection, target molecules and acupuncture time are to establish for exploration of adenosine release and the change rule of effects of acupuncture. The expected results of the project are to further clarify and improve the material basis of theory of traditional acupuncture and effects of acupuncture.

中医针灸领域的针刺效应物质基础假说引起了当今科学界的广泛关注，而对针刺效应物质释放和转化规律的认识国内外还一筹莫展，这大大限制了针灸医学的发展和人们对针灸本质的科学认识。本申请项目创造性地运用功能化的表面增强拉曼光谱（SERS）原理和检测方法来动态探测腺苷类针刺效应物质的释放与转化。利用磁控溅射纳米技术，发展夹层含有内标信号分子的核壳式SERS基底的合成方法和原理，并在此表面进行功能化设计，制备出集针刺、采样和信号放大于一体的新型基于针灸针的SERS活性探针;选择大鼠作为研究对象，通过复制膝骨性关节炎动物模型，开展对针刺效应物质研究，实现对针刺效应物质腺苷类目标分子定量和选择性动态SERS检测;建立SERS光学检测信号、目标分子与针刺时间的量效关系，探索腺苷类针刺效应物质释放和变化规律。本项目的预期成果为进一步明确和完善传统针灸学针刺效应的物质基础理论。

传统针灸学源远流长，是我国医学科学的特色和优势, 并对世界医学发展产生了积极的影响。然而针灸并没有给出明确的现代科学依据，针灸作用机理不明确，这很大程度上限制着针灸的发展和推广，也是针灸在国内外并没有受到广泛认可和接受最主要原因。这大大限制了针灸医学的发展和人们对针灸本质的科学认识。本项目针对以上问题开展了(1)新型修饰针灸针SERS活性基底的设计与制备；(2)选择性检测SERS探针的设计原理与方法研究；(3)新型修饰针灸针SERS探针对针刺效应物质的动态SERS检测。研究获得了系列成果。包括：(1)设计了两亲功能化针灸银针传感器，实现了原位多相体系的多种物质的检测；(2)研制出基于铁-配位表面增强共振拉曼光谱标记探针，实现了对儿茶酚胺神经递质的探究与分析；(3)构建出基于功能化的针灸针的SERS探针，实现对复杂体液中多巴胺的快速检测；(4)设计并构建出基于功能化针灸针SERS活性平台，完成对三磷酸腺苷的快速检测；(5)完善高亲和力Fe3O4/Au探针，进一步实现高灵敏检测生物样本中多巴胺；(6)针对其他体系，设计新型探针并开展其他方面的应用研究。通过针灸针探针的优化设计，实现复杂体液条件下的多种针刺效应物质比如多巴胺、肾上腺素、三磷酸腺苷等物质的快速探测。本项目的预期成果为进一步明确和完善传统针灸学针刺效应的物质基础理论。