超声波辐照诱导心肌正性肌力作用的实验研究

As we knewn that ultrasonic irradiation might increase the membrane permeability of myocardial cell and change the function of trans-membrane ion transport channel, and would lead to an obvious change of depolarization and repolarization of membrane and inner concentration distribution of force related ions of myocardial cells finally. .The existed biological effect of ultrasound irradiation might impact on the contractility of myofibrils within myocardial cells during systole and finally induce a change of myocardial contraction force in different ways regionally and globally. .This study intends to use patch clamp techniques combined with synchronized laser confocal microscope technology and ultrasonic tissue Doppler and speckle tracking imaging techniques to observe the diversity of myocardial contractility under different parameter settings of ultrasonic irradiation systematically in different levels: .1. Microscopic level: the simutaneous changes of membrane permeability and force of single myocardial cell, and the changes of ion channels function and inotropic action related inner ion concentration and distribution. .2. Macroscopic level: the changes of ultrasound visualized regional and global myocardial mechanics during action potential mapping of group myocardial cells. .3. To establish the co-relationship between the membrane permeability of myocardial cell, functional changes of ion channel and mechanics of single myocardial cell and group myocardial cells spatio-temporally. .Based on the potential research results of ultrasonic irradiation induced myocardial mechanical re-function to explore and establish the necessary conditions and optima of ultrasound irradiation parameter settings for an effective regional and global positive inotropic action, and to fund a basic experimental dataset and an innovative method for the further development of clinical non-invasive physical therapeutic method of heart failure ultimately. .Our research team has performed a pilot study and set up a file in the above research areas for this proposed experiment in the past few years.

超声波辐照有可能造成心肌细胞膜通透性和离子转运通道功能发生改变，从而影响心肌细胞膜除极和复极过程以及细胞内肌力相关离子浓度分布。超声波的上述生物学效应将影响心肌细胞内肌原纤维收缩功能并导致不同的肌力改变。本研究拟采用膜片钳技术结合同步激光共聚焦显微镜技术以及超声组织多普勒和斑点跟踪成像等技术在不同层面系统性观察不同参数超声波辐照环境下：1.微观的单个心肌细胞膜的通透性和离子通道功能变化与单个细胞肌力及其内相关离子浓度分布变化；2.宏观的细胞群单向动作电位标测结果与整体细胞群力学功能状态变化；3. 明确单个细胞及心肌组织的心肌细胞膜通透性和离子通道功能改变与力学功能状态变化间的时空关系和机制。基于以上研究结果建立超声波辐照诱导心肌正性肌力作用的必要参数条件和优化参数条件，为建立全新的无创物理性心力衰竭治疗方法提供基础实验数据。近年来本课题组已进行相关预实验和前期研究。

超声波辐照有可能造成心肌细胞膜通透性和离子转运通道功能发生改变，从而影响心肌细胞膜除极和复极过程以及细胞内肌力相关离子浓度分布。超声波的上述生物学效应将影响心肌细胞内肌原纤维收缩功能并导致不同的肌力改变。本研究拟采用膜片钳技术结合同步激光共聚焦显微镜技术以及超声组织多普勒和斑点跟踪成像等技术在不同层面系统性观察不同参数超声波辐照环境下：1.微观的单个心肌细胞膜的通透性和离子通道功能变化与单个细胞肌力及其内相关离子浓度分布变化；2.宏观的细胞群单向动作电位标测结果与整体细胞群力学功能状态变化；3. 明确单个细胞及心肌组织的心肌细胞膜通透性和离子通道功能改变与力学功能状态变化间的时空关系和机制。基于以上研究结果建立超声波辐照诱导心肌正性肌力作用的必要参数条件和优化参数条件，为建立全新的无创物理性心力衰竭治疗方法提供基础实验数据。