可注射天然聚酯支架的仿生功能设计及医美微整形骨修复研究

Due to the particularity and complexity of medical cosmetology, traditional natural biomaterials are difficult to meet the multiple requirements of structure and function, which restricts their development. In this project, based on the concept of In Situ Induced Regeneration, the main material of natural biomaterial polyhydroxyalkanoate (PHA), and phospholipid-modification technology for fusion of proteins-materials, the novel injectable PHA microspheric scaffolds with open porous structure, tissue fusion, osteo-induction, long-term anti-infection and high bone imitation structure will be designed and prepared. We will firstly study the effect of phospholipids-protein proportion on the structure and function of PHA microspheric scaffolds. At the same time, the properties and mechanism of tissue integration, in situ osteo-induction and antibacterial performance will be evaluated. Finally, the PHA microspheric scaffold will be transplanted into rat skull defect model by minimally invasive injection to determine the capabilities of tissue integration, osteo-induction and anti-infection for inducing bone regeneration. This study aims to establish a convenient, safe and effective new method to induce in situ bone regeneration, which will lay theoretical basis for the functionalization of PHA and its application in medical cosmetology.

由于医美微整形的特殊性和复杂性，传统的生物材料难以同时满足“从结构到功能”的多项需求，制约了其发展。针对该问题，本项目借鉴“原位诱导再生”理论，以天然生物材料聚羟基脂肪酸酯（PHA）为主体材料，利用磷脂修饰技术促进功能蛋白（生长因子或抗菌肽）与材料融合，设计制备兼具开放式多孔结构、组织融合、原位骨诱导及长效抗感染的多功能可注射PHA微球支架。首先探索磷脂与功能蛋白比例对微球支架结构及功能的影响，考察基于“仿生结构和原位诱导再生”双机制增强骨组织整合的构效关系，对微球支架的原位骨诱导-抗感染协同能力进行评价并解析其机理。最后将PHA微球支架经微创注射移植于大鼠颅骨缺损模型，确定该PHA微球支架应用于医美微整形骨诱导组织再生和功能重建的科学性和可行性。本研究旨在建立一种更方便、安全、有效的原位诱导骨再生的新方法，也为PHA材料功能化及其医美微整形研究奠定理论基础。

由于医美微整形的特殊性和复杂性，传统的生物材料难以同时满足“从结构到功能”的多项需求，制约了其发展。我们针对该问题，借鉴了“原位诱导再生”理论，以天然生物材料聚羟基脂肪酸酯（PHA）的最新成员PHBVHHx为主体材料，利用磷脂修饰技术促进功能蛋白（生长因子或抗菌肽）与材料融合，成功地设计制备兼具开放式多孔结构、组织融合、原位骨诱导及长效抗感染的多功能可注射PHA微球支架（H-B-OPM），也发现了“仿生结构和原位诱导再生”双机制能增强骨组织再生，并证实了基于本微球支架的原位骨诱导-抗感染存在显著的协同能力。成功将H-B-OPM经微创注射移植于小鼠异位成骨模型，具有应用于医美微整形骨诱导组织再生和功能重建的价值，也为PHA材料功能化及其医美微整形研究奠定理论基础。