外源激素对应力木形成调控机理的研究

It has been being the hot and multi-disciplinary topic related to wood science,tree physiology, plant botany, forest breeding and silviculture and wood technology to study the reaction wood formation controlled by exogenous hormone. In this project, the most important fast growing species, masson pine and poplar seedling plantation, are selected as material.we try to culture the seedling of masson pine and poplar and then lean their stem horizontally to form the compression and tension wood in the same conditons. The tree growth feature, the micro-structure and cell wall characteristics of the seedling which were treated by different exogenous hormones (IAA and GA3) with different concentrations are determined successively and their varation patterns are analyzed and compared. The effect of different kinds of exogenous hormones and concentration on the growth feature, the micro-structure and cell wall feature of reaction wood of the leaning seedlings are clarified, the relationships between the different kinds of exogenous hormones and concentraions,and the tree morphyology, the micro-structure and cell wall characteristics, and the mechanism of reaction wood formation controlled by exogenous hormone are revealed. These results will provide the scientific information for effectively controlling the reaction wood formation, reducing defects of wood processing and utilization, improving the quality and utilized ratio of wood in forest silviculture and utilization of fast growing plantation.

外源激素调控应力木形成的研究一直是木材科学，树木生理学，植物学，遗传育种学，森林培育学和木材加工利用学等多学科交叉前沿的研究热点和难点。本项目拟马尾松和杨树为对象，在相同生长条件下人工培育、水平倾置马尾松和杨树苗木，通过对比施加不同浓度的外源激素（IAA和GA3），连续跟踪测定分析马尾松和杨树树干的生长形态特征，木材细观构造及细胞壁特性的定量变化和演变规律，弄清不同激素种类和浓度对水平倾置马尾松和杨树应力木生长形态特征，形成区和对应区的宏观特征，细观构造及细胞壁特性的影响，探明外源激素种类、含量与应力木形态特征，细观构造和细胞壁特性之间的相关关系，揭示外源激素对应力木形成的调控机理，从而为在人工林营林培育和加工利用中有效地控制应力木的产生、减少应力木缺陷造成的损失、提高木材品质及利用率提供理论依据。

外源激素调控应力木形成的研究一直是木材科学，树木生理学，植物学，遗传育种学，森林培育学和木材加工利用学等多学科交叉前沿的研究热点和难点。本项目拟以我国重要速生用材树种人工林杨树和马尾松为对象，从在相同环境和立地条件下人工培育和水平倾置苗木来模拟应力木形成过程入手，通过在生长季节通过对比施加和不施加不同浓度的外源激素为突破口，连续跟踪测定分析外源激素处理后应力木生长性状和细胞壁构造特征的定量变化和演化规律，弄清生长过程中外源激素对马尾松和杨树应力木的生长特征，应力木形成区和对应区解剖特征、超微构造和胞壁特性的影响及其相互关系，揭示外源激素对马尾松和杨树应力木形成的调控机理，从而为在人工林营林培育和加工利用中有效地控制应力木的产生、减少加工利用缺陷、提高木材品质及利用率提供理论依据。经过四年的测定研究和分析，较好的完成了预期的研究计划，达到了预期的研究目标。通过研究探明了施加不同浓度的外源激素（IAA和GA3）对水平倾置杨树和马尾松应力木生长形态特征，形成区和对应区的宏观特征，细观构造及细胞壁特性的影响；成功建立了固相萃取-高效液相色谱法（SPE-HPLC）测定杨树内源激素IAA的方法和利用ImageJ软件测量胶质层面积比率的方法；探明了外源生长素（IAA）及其抑制剂NPA对生长素极性运输基因（AUX2、PIN1和ABCB1）和纤维素生物合成基因（CesA4、CesA5、CesA7、CesA8、CesA13、CesA17、CesA18、SUS1、SUS2、UGP1和UGP2）表达的影响；揭示了外源激素种类、含量与杨树和马尾松应力木形态特征，细观构造和细胞壁特性之间的相关关系；依托本基金项目培养研究生2名，博士后1人，在国外SCI/EI收录期刊发表1篇，在国内重点核心期刊发表论文2篇，国内外学术会议上交流会议论文3篇，另有2篇英文文章已整理完毕待发表。