肾虚血瘀型膝OA力学微环境稳态的软骨阻尼特性研究

The knee osteoarthritis is merely among middle aged and elderly people whose symptoms are usually renal deficiency and blood stasis. Based on the preliminary study, renal deficiency and blood stasis type knee has been constructed, osteoarthritis musculoskeletal model, combining bone, cartilage, ligament and muscle as well. The form study also discovered the stress distribution’s nonlinear characteristic of "ligament" and "bone" in knee, and cartilage repair needs stable dynamical microenvironment. Based on the nonlinear characteristic mentioned above, this project will construct solid simulation model with the adoption of biomechanical test and reverse engineering. By testing the model with sliding slope, contact area and lubricant experiment, the study would get the optimum intervention data and cartilage damping parameter of knee osteoarthritis’s microenvironment. With the help of compounded knee osteoarthritis model, renal deficiency model, blood stasis model to the renal deficiency and blood stasis animal model, the study would loaded revaluated data to animal model. Cartilage repairing condition would be analyzed and cartilage damping would be verified according to biochemical index, pathological section,gene expression and gait analysis as assessment methods. On the basis of biotic coupling theory, we will demonstrate the cartilage damping characteristic in dynamical microenvironment. In this way, the study proves the nonlinear characteristic of cartilage damping in dynamical microenvironment, elucidates homeostasis characteristic of cartilage damping in dynamical microenvironment and finally reveals the mechanism of cartilage damping in dynamical microenvironment. This project will provide objective solid model for the "emphasis on both sinews and bones", and give quantitative diagnosis evidences as well as qualitative treatment approaches for the diagnosis and treatment of renal deficiency and blood stasis type knee osteoarthritis.

膝骨关节炎（OA）以中老年为主，肾虚血瘀型多见。前期研究构建了肾虚血瘀型膝OA集骨、软骨、韧带和肌肉的肌骨模型，发现了膝部"筋""骨"应力分布非线性特征，软骨修复需要力学微环境稳态。本项目通过"筋""骨"应力非线性特征，采用生物力学测试、逆向工程技术构建仿真实体模型。对实体模型行滑动坡度、接触面积、润滑液干预实验，得出膝OA力学微环境稳态的最佳干预数据和软骨阻尼参数。采用膝OA模型、肾虚模型、血瘀模型，复合成肾虚血瘀型动物模型。把换算后干预数据加载于动物模型，运用生化、病理、基因表达、步态分析评价软骨修复程度和验证软骨阻尼。结合生物耦合理论，对力学微环境稳态软骨阻尼特性进行论证，证明力学微环境稳态软骨阻尼非线性特点，阐明力学微环境稳态软骨阻尼动态平衡特征，揭示力学微环境稳态的软骨阻尼特性机制。为研究"筋骨并重"理论提供客观实体模型，为临床诊治肾虚血瘀型膝OA贡献定量诊断证据和定性治疗思路。

膝骨关节炎（OA）以中老年为主，肾虚血瘀型多见。前期研究构建了肾虚血瘀型膝OA集骨、软骨、韧带和肌肉的肌骨模型，发现了膝部"筋""骨"应力分布非线性特征，软骨修复需要力学微环境稳态。.本项目运用来自前期研究所获取的数据作为三维重构的输入数据，采用生物力学测试、逆向工程技术，结合大量断层二维图像，选取适当的分割阈值提取需要重建的组织结构轮廓，实现对计算机断层摄影技术产生系列断层图像重建三维实体模型,对实体模型行滑动坡度（运用肾虚血瘀型膝OA肌肉-骨-软骨仿真实体模型采用两个不规则表面的软骨试样，行滑动坡度对关节软骨阻尼特性影响实验）、接触面积（运用肾虚血瘀型膝 OA 肌肉-骨-软骨仿真实体模型，通过改变外载荷和滑动速率来研究软骨摩擦副的接触状态和摩擦特性，行接触面积对关节软骨阻尼特性影响实验）、润滑液干预实验（运用肾虚血瘀型膝 OA 肌肉-骨-软骨仿真实体模型，采用三种不同的润滑环境对关节软骨阻尼特性影响实验）得出膝OA力学微环境稳态的最佳干预数据和软骨阻尼参数。.采用经典的 Hulth 造模法建立膝骨关节炎模型，去卵巢法建立肾虚模型，肾上腺皮质激素和肾上腺素法建立血瘀模型。复合模型由上述 3 种模型复合而成。把仿生实体模型膝 OA 力学微环境稳态软骨阻尼的非线性参数经过换算后干预数据加载于肾虚血瘀型膝 OA 动物模型，加载干预数据后，分别进行滑动坡度；接触面积；润滑液体对关节软骨阻尼的影响实验；运用生化指标、病理切片、基因表达、步态分析评价软骨修复程度和验证软骨阻尼。结合生物耦合理论，对力学微环境稳态软骨阻尼特性进行论证，证明了力学微环境稳态软骨阻尼非线性特点，阐明了力学微环境稳态软骨阻尼动态平衡特征，揭示了力学微环境稳态的软骨阻尼特性机制。为研究"筋骨并重"理论提供客观实体模型，为临床诊治肾虚血瘀型膝OA贡献定量诊断证据和定性治疗思路。