毫米波特异反应基因的探索及研究

BACKGROUND AND OBJECTIVE: Numerous publications have reported that over 50 diseases and conditions have been treated successfully with millimeter wave (MW) alone, or in combination with other treatment modalities in over 3 million patients in the former USSR. We previously found that MW has opposite effects on GJIC from extremely low frequency magnetic fields and MW could eliminate the suppression of GJIC induced by TPA. However, it is unclear about the mechanisms of the biological effects elicited by MW exposure. In the present study, Genechip analysis, a high-through technology was employed to investigate gene expression pattern in response to MW exposure. METHODS: An Affymetrix human genome U95 GeneChip was employed to quantify the gene expression level after the keratinocyte cell exposure to MW. Then gene expression level was validated by RT-PCR. RESULTS: The data showed that totally 18 genes were up-regulated more than 3 times in response to MW stimulation. Among them, 2 genes (Human proteinase-activated receptor-2 gene, PAR-2; Human melanoma antigen recognized by T-cells gene, MART-1) were of particular interest and validated by RT-PCR. CONCLUSIONS: MW could stimulate keratinocyte cells by changing mRNA level of related genes. These genes may be related with immune modulation and signal transduction of the cell.

本项目针对毫米波临床应用发展中存在着机制不清的问题而提出。.首先确定有效的毫米波涮跫ㄒ鹣赴硇透谋洌辉诖嘶∩希肗orth.ern法观察EMF CA及EMF.CB二个基因与毫米波的相关.性.，以及采用差异显示反转录法（DD），揭示毫米波辐照的特异反应基因，从而在基因水平认识毫米波的作用机制。这对指导毫米波.的临床应用具有重要的理论和导室庖濉?.