小麦全蚀病菌钙离子泵GgVCX1和GgPMC1基因分子致病机理

Wheat Take-all disease caused by Gaeumannomyces graminis var. tritici, spreads rapidly and seriously affects wheat production. Due to the limitations of the materials and methods, people don’t have enough knowledge of the pathogen genetic background as well as the molecular mechanism of pathogenicity. Calcium ion pump Vcx1 and Pmc1 is an important transport protein fungi calcium signal pathway, when fungi infect plant hosts. Earlier, we had established stable T-DNA mutant library and transcriptome sequencing method.obtained inserted mutant (G38) of GgVCX1 gene. Transcriptome analysis of the data showed a differential expression of related genes in many calcium signal channels (GgVCX1/GgPMC1), indicating that the expression is associated with pathogenicity. However, there is no study on calcium signaling pathway in the plant pathogenic fungi G. graminis , and no associated outcome of this kind of study reported as well. Therefore, in this study, the roles of Calcium ion pump pathway in the process of infection by G. graminis GGT-007, especially GgVCX1/GgPMC1 genes, will be studied by using Real-Time PCR、inhibitor analysis、gene deletion and subcellular localization in wheat take-all fungus. The outcome of this study will provide a foundation for deciphering the molecular mechanism of calcium signal pathway, and understanding the growth and development, controling pests of wheat take-all.

小麦全蚀病（Wheat Take-all）是一种毁灭性病害。钙离子泵Vcx1和Pmc1是真菌钙离子信号调控侵染及其致病的重要转运蛋白。课题组前期利用农杆菌介导转化全蚀病菌获得了GgVCX1基因插入突变体（G38），其对小麦全蚀病菌表观特性及致病性都有显著影响，在转录组测序侵染全蚀病菌小麦根部试验中显示钙离子泵GgVCX1和GgPMC1基因上调表达，预示这两个钙离子泵基因与侵染致病相关，有待于深入研究。但在小麦全蚀病菌这一根部病害中钙离子信号通道研究滞后，未见相关报道。因此，本项目拟从钙离子泵GgVCX1/GgPMC1基因着手，利用实时定量PCR、基因敲除、突变体互补、亚细胞定位等技术解析GgVCX1和GgPMC1基因在全蚀病菌侵染致病过程中生物学功能，分别从细胞、分子、生化和基因表达等方面探明其介导钙离子信号过程。以期阐明全蚀病菌钙离子泵在侵染致病过程中作用，为小麦全蚀病防控策略提供依据。

由禾顶囊壳菌小麦变种引起的全蚀病（Take-all）是一种小麦毁灭性病害。钙离子泵Vcx1和Pmc1是真菌钙离子信号途径调控侵染及其致病的重要转运蛋白。本项目首次利用Crispr/cas9技术，开展小麦全蚀病菌遗传转化研究，为小麦全蚀病菌的基因敲除等分子生物学分析提供了可靠的依据。从新颖的材料（小麦全蚀病菌致病性降低的突变体）和视角VCX1/PMC1基因（Ca2+泵）着手，分别从细胞、分子、生化和基因表达等方面初步探明了其介导的钙离子信号过程,初步解析了全蚀病菌VCX1/PMC1基因致病性的分子机制及其特异性，在小麦全蚀病菌致病机理研究方面获得了重要的成果。