牛磺酸对视杆细胞功能及视杆通路信号传导影响的研究

Taurine is held to function as a neuromodulator and osmoregulator in the central nervous system, and play a major role in modulating excitatory pathways. The increasing evidences indicate that taurine has many functions in retina, however, there are still many problems unknown. After dietary supplementation of taurine in weanling rats, we found that the expression of Gαt1 in rat retina increased, taurine affected retinal glutamate[Glu] andγ-aminobutyric acid[GABA] contents, and significantly increased the mRNA levels of mGluR6, ρ1、ρ2 subunits of GABAcR in retina. We also studied the effects of taurine on light and dark adaptations, the results showed that Gαt1 were mainly located on the outer segments of rods, the retinal contents of Glu significantly increased and the mRNA levels of mGluR6 decreased in dark adaptation, whereas the mRNA levels of GABAcR ρ1、ρ2 subunits did not change. Taurine only effected the localization and expression of iNOS in dark adaptation. Our study demonstrates that tauine plays important roles in dark adaptation function, taurine can regulate the photo-transduction of the rod cells, taurine also can modulate rod visual signal transmission by affecting the retinal contents of Glu, GABA and the expression of Gαt1、mGluR6、GABAcR and iNOS in retina. Our works may be helpful to understand the roles of taurine as a neuromodulater in retina.

通过给断乳大鼠补充牛磺酸，观察牛磺酸对视杆细胞功能及光转导蛋白Gatl的影响，探讨在视网膜上牛磺酸与Glu，GABA及其受体在定位、分布和表达上的关系，以及牛磺酸对视杆通分蠫lu，GABA与其相应受体mGluR6，GABAcR的信号耦联的影响，旨在深入阐明牛磺酸影响视杆细胞功能及视觉信号传导，尤其是视杆通路信号传导的分子机制。