磁性微球-聚醚类药物抗体复合物制备及性质研究

Adopt double fine emulsion polymerization, the morphology rules, the preparation of magnetic response is good, with surface of carboxyl functional groups of magnetic polymer microsphere. To polyether drugs molecule structure transform, preparation half an antigen, and OVA or BSA connection, to form a completely antigen, immune animals, preparation of monoclonal antibodies. Preparation organic silicon doped phenanthroline ruthenium compound, inorganic silicon coated with the combination of double structure fluorescence silica microspheres nano. Use of magnetic nanoparticles spheres carboxyl surface with antibody surface amino reaction, make magnetic nanoparticles microspheres connected with antibody preparation immune magnetic nanoparticles microspheres; Using fluorescence microspheres surface active functional group (carboxylic), use lively ester method will veterinary medicine monoclonal antibody accidentally united microspheres surface, preparation immunofluorescence microspheres. To get immune magnetic nanoparticles microsphere and immunofluorescence micro ball purification and the nature of the study, with magnetic microsphere as separation carrier, fluorescence microspheres as probes, a biological samples for drug residue detection of fluorescence immune detection method, and the actual samples for testing..The significance of this research lies in new magnetic nanoparticles and fluorescence microspheres into veterinary drug residue research fields, to explore the veterinary drug residue test new technology provide the theoretical basis.

采用双细乳液聚合法，制备出形貌规则、磁响应性好、表面含有羧基功能基团的磁性高分子微球。对聚醚类药物分子进行结构改造，制备半抗原，与OVA或BSA连接后，形成完全抗原，免疫动物，制备单克隆抗体。制备有机硅掺杂菲咯啉联钌，无机硅包覆相结合的双结构荧光二氧化硅纳米微球。利用磁性纳米微球的表面羧基与抗体表面的氨基反应，使磁性纳米微球与抗体相连制备免疫磁性纳米微球；利用荧光微球表面的活性功能集团（羧基），采用活泼酯方法将兽药单克隆抗体偶联在微球表面，制备免疫荧光微球。对获得的免疫磁性纳米微球和免疫荧光微球进行分离纯化并对其性质进行研究，以磁性微球作为分离载体、荧光微球作为探针，建立检测生物样本中药物残留的荧光免疫检测方法，并对实际样本进行检测。.本研究的意义在于将新型磁性纳米颗粒和荧光微球引入兽药残留研究领域，为探索兽药残留检测新技术奠定理论基础。