基于代谢组和蛋白组学对大鼠射精机理脑神经物质基础的研究

Sexual behavior is an important factor in the harmony of couples. At present, 25% of men in China have sexual dysfunction, which seriously affects family happiness. Ejaculation is the strongest component of sexual activity, but its mechanism is extremely complex, not yet clear. Studies have shown that the brain is the "headquarters” of ejaculation, and is the result of coordinated control of multiple brain regions and multiple neurotransmitters. However, there is still no systematic study of the material basis during ejaculation process. Our team have carried out research on transcriptomics in the early stage and found for the first time that ejaculation is closely related to change of gene expression, but clarifying the mechanism is still lacking in material basis. Therefore, this project intends to carry out metabolomic and proteome sequencing analysis of the brain nervous system during the ejaculation of male rats, to explore the significant differences in ejaculation-related metabolites and proteins, and establish a protein-metabolite interaction network for the molecular biology mechanism of ejaculation exploring new research approaches, and has potential value in the diagnosis, treatment, and drug development of male sexual dysfunction.

性行为是夫妻和谐的重要因素，目前我国有25%的男性有性功能障碍，严重影响家庭幸福，而射精是性行为中最强烈的组成部分，但其机理极其复杂，尚不明确。研究表明，脑是射精的“司令部”，且是多个脑区及多种神经递质互相调控的结果。然而，目前仍然没有系统化研究射精过程的物质基础。我们团队在前期开展了转录组学的相关研究并首次发现，射精与基因的表达变化紧密相关，但阐明作用机制仍缺乏物质基础。因此，本课题拟对雄鼠射精发生过程脑神经系统性地进行代谢组和蛋白组测序分析，挖掘与射精相关的显著差异代谢物和蛋白，构建蛋白—代谢物互作调控网络，为阐释射精的分子生物学机理探讨新的研究途径，对男性性功能障碍的诊断、治疗以及药物研发具有潜在的重要价值。

性行为是夫妻和谐的重要因素，目前我国有25%的男性有性功能障碍，严重影响家庭幸福，而射精是性行为中最强烈的组成部分，但其机理极其复杂，尚不明确。研究表明，脑是射精的“司令部，且是多个脑区及多种神经递质互相协调控的结果。然而，目前仍然没有系统化研究射精过程的物质基础。基于HNMR代谢组学方法进行代谢组学和DIA定量蛋白质组学对雄鼠射精过程进行开展研究。代谢组学研究发现共有20个标志性代谢物发生显著性变化，三条代谢通路Alanine, aspartate and glutamate metabolism，D-Glutamine and D-glutamate metabolism和Arginine Arginine and proline metabolism可能参与射精调控；蛋白质组学研究发现，2条信号信号通路AMPK和突触信号通路，以及挖掘出5个标志性蛋白分别为Akap17a、Marcksl1、Rab11a、Kng1、Fn1。Akap17a、Marcksl1和Rab11a在射精时显著上调表达，而Kng1和Fn1显著下调表达，可能参与射精调控。为阐释射精的分子生物学机理提供可能的物质和信号通路基础，对男性性功能障碍的诊断、治疗以及药物研发具有潜在的重要价值。