用于胃癌原位诊断的靶向性纳米增强拉曼光谱技术

Surface enhanced Raman spectroscopy (SERS) has low interference from background noise comparing to other spectral techniques, and can obtain specific molecular spectral information with unique and clear fingerprint characters and therefore possesses great advantages in aqueous environment. Nanoparticle enhanced Raman spectroscopy can further improve the stability, universality and applications of SERS, especially in biological systems. Here we develop a Raman spectroscopy system integrated with aptamer conjugated SERS nanoparticles, Raman micro-probe and Raman spectrometer for in-situ Gastric cancer diagnose. Our research mainly includes several subjects: 1) Synthesize core-shell nanoparticles with high SERS activity and stability, and conjugated with aptamer to develop a unique bio-recognition substrate; 2) Design a special Raman micro-probe coupled with bio-recognition substrate, and assemble with a Raman spectrometer to construct a complex Raman spectrometric system; 3) Develop a real-time and in situ diagnosis method by comprehensive chemometrics and software algorithms in analyzing Raman spectrum of gastric cancer cells. We expect to develop a specific Raman system to collect and analyze Raman spectra of cancer cells, and can further provide important insights and technology for real-time and in-situ gastric cancer diagnose.

表面增强拉曼光谱技术（SERS）具有低噪音和背景干扰、高灵敏度和可获得指纹图谱等特点，纳米材料增强拉曼光谱技术可以进一步提高SERS技术的稳定性、普适性以及拓展其在生物体系的应用。针对肠胃癌症等高死亡率疾病快速检测方法的重大需求，本项目拟通过在核壳结构拉曼增强纳米粒子表面修饰具有高特异性识别功能的核酸适体（Aptamer）分子，结合特殊设计的拉曼微探头和光纤拉曼光谱仪器，研究并开发可对胃癌组织进行实时原位诊断的技术，拟开展以下研究： 1）合成高SERS活性和稳定性的核壳结构纳米粒子，并结合Aptamer分子形成具有特异识别功能的生物拉曼探针； 2）设计微型拉曼光学探头并集成特异识别拉曼探针以及拉曼光谱仪搭建完整的检测系统；3）通过化学计量学及软件算法开展胃癌细胞的拉曼谱图分析研究。项目研究结果可望实现对胃癌细胞拉曼信号的快速采集与识别，并为胃癌的实时原位诊断方法开发提供理论和实验依据。

表面增强拉曼光谱（SERS）具有高灵敏度、指纹识别能力和耐光漂白等优势，在复杂生物检测和原位化学反应监测等领域有着广泛的应用前景。研究工作以多层核壳结构纳米粒子、金纳米粒子组装体为拉曼增强活性基底，结合高特异性核酸适体分子，研究对癌细胞的靶向识别和SERS成像，并实现表面修饰纳米粒子对目标细胞的靶向性结合。另外，近年来拉曼测试仪器与技术在储能研究方向中应用蓬勃发展。本项目设计了一种包覆式自支撑电极，优化了透明柔性电池封装，搭建了原位拉曼电化学测试平台，可提供电池性能测试无法给出的电极结构微形貌、组成、机械力演化等数据，有望广泛应用于新型活性材料的机理解释和设计优化。