蚕豆单宁相关基因的分子标记及定位研究

Faba bean (Vicia faba Linn) is one of the most important products for export and a vital feedstuff of plateau graziery. Nevetheless, main cultivars in Qinghai province possess tannin that damage the nutritions of faba bean. Tannin which exist in faba bean brings serious effections on commodity rate and reduces the usage of protein feed. Thus, it is of significant for breeding faba bean without tannin. In this study, a total of 426 F2 plants and F2:3 progenies derived from Qinghai12 × 3290 were used to develop linkage maps of genes related with tannin using SSR markers. The linkage maps and SSR markers in this study may provide a sound basis for further breeding of faba bean without tannin, and should be useful in marker assisted selection (MAS) and cloning of the genes.

蚕豆是青海省出口的特色农产品之一，也是高原畜牧业的重要饲料原料。然而目前青海省主栽的蚕豆品种中含有抗营养因子（单宁）。蚕豆中单宁的存在严重影响了蚕豆的商品率并且降低了蚕豆作为蛋白质饲料的利用率。选育无单宁蚕豆品种已成为蚕豆品质育种的重要方向，但通过常规育种选育无单宁蚕豆品种难度较大。本研究拟选用由无单宁蚕豆材料"3290"和高单宁品种"青海12号"杂交得到的含426个单株的F2及F3分离群体为作图依据，并应用SSR标记筛选与蚕豆单宁基因紧密连锁的分子标记，从而完成对蚕豆单宁相关基因的作图和定位。为蚕豆无单宁品种的选育提供理论依据，为进一步进行该基因的分子标记辅助育种和基因的克隆奠定基础。