花青素苷在红山茶组植物化学分类中的意义研究

There are 57 species with red petals in the Section camellia, which are either famous ornamental flowers plant in the world. Study on taxonomy of this Section have been widely reported based on morphology, cytology, secondary metabolism, molecular analysis, etc., but the results of taxonomy had still greater conflicts among different methods. It is important and urgently to be solved that how to combine all these methods and results, and to make a comprehensive analysis to provide a cogent conclusion in taxonomy of Sect. camellia. .A new convincing classification will be studied using most species of the Sect. camellia and many species of other Sections in the Genus camellia in this project. Qualitative and quantitative analysis of flavonoids such as anthocyanins and flavonols in the petals of these species will be analysis by HPLC. Also, the principal component analysis will be performed for the composition and the content of the flavonoids by SAS software, as well as a cluster analysis will be performed based on the Euclidean distances computed using ward 's minimum variance analysis, and the dendrogram of all species will be drawn using PROC TREE of the SAS system. Petals and young leaves will be used to extract DNA. PCR amplification of the ITS1-5.8s-ITS2 regions of the nrDNA and matK will achieve. PCR products will clone and sequencing. The gene tree will generate using a maximum likelihood algorithm. More valuable results on taxonomy of Section camellia will be obtained by overview the chemical taxonomy based on flavonoids and nrITS sequence combined cytology and geographical distribution. The differences and disputes on the origin, speciation, dispersal and evolution trend of the Section camellia will be resolve by our research. This importance and guidance value of anthocyanin in taxonomy and species delimitation of Section camellia will be confirmed by this project. This will serve as a theoretical underpinning for the protection and exploitation of anthocyanins in the petals of the Section camellia species.

红山茶组植物有57个种，花瓣红色，是世界著名的观赏花卉。一些学者以基于形态学、细胞学、次生代谢成分和基因结构等为依据对其进行分类，结果存在较大分歧。如何将众多分类依据和结果集合起来进行综合评价，得出更有说服力的分类结论，是红山茶组植物分类中急需解决的问题。 .项目拟采集红山茶组植物绝大多数物种，用HPLC技术分析花瓣中的花青素苷等黄酮类成分，SAS分析软件对结果进行主成分分析，ward’s最小方差遗传聚类分析；同时提取各物种花瓣和幼叶DNA，进行nrITS和matK区域的扩增、克隆和测序，用最大似然和简约法进行聚类分析，生成进化树。结合物种细胞学和地理分布，综合评价实验结果，获得更具有说服力的分类结论，以解决红山茶组植物分类中在物种起源、分化、扩散路线和进化趋势等方面的分歧和争议。同时，确定花青素苷在分类和物种鉴定中的重要价值和指导意义，为红山茶组植物种质资源的保护与合理开发应用奠定基础

红山茶组植物是世界著名的观赏花卉，有57个种，花瓣红色。一些学者基于形态学、细细胞学、次生代谢成分和基因结构等为依据对其进行分类结果，存在较大分歧。如何将众多分类依据和结果集合起来进行综合评价，得出更有说服力的分类结论，是红山茶组植物分类中急需解决的问题。.项目立项后，到9个红山茶组植物自然分布省份考察了8次，采集49个物种花朵、叶片和果实；用HPLC技术分析了花瓣花青素苷、黄酮等类黄酮的组成和含量，通过SAS分析软件的主成分分析，ward’s最小方差遗传聚类分析建立了系统树进行分类研究；用试剂盒提取各物种花瓣和幼叶DNA，进行nrITS和matK序列扩增、克隆和测序，用最大似然和简约法进行聚类，生成进化树。结合物种细胞学和地理分布结果，在云贵川的长江上游至西江上游处，红山茶组植物有明显的演化，物种分布丰富，为解决红山茶组植物分类中在物种起源、分化、扩散路线和进化趋势等方面的分歧和争议，以及为红山茶组植物种质资源的保护与合理开发应用奠定基础。