纤维鞘FSCB/CABYR蛋白复合物参与精子运动和获能调节的分子机制研究

It is well-known that many of the capacitation-associated changes are depend on the cAMP/PKA signal cascades and meanwhile the initiation of hyperactivation is Ca2+-dependent. However, the relationship between the cAMP/PKA signal cascades and Ca2+ signal is still not clear. The applicant found a novel FSCB/CABYR protein complex on the fibrous sheath for the first time, which not only participated in the transduction of the cAMP/PKA signal cascades, but also has the capability of Ca2+-binding. Therefore, this study will Base on the existing of FSCB knockout mice, both the FSCB and CABYR knockout mice will be constructed and the effects of the deficiency of the FSCB/CABYR complex on the sperm movement and capacitation will be detected and discussed. This research not only has important theoretical significance for understanding the molecular mechanism of capacitation, but also could provide a novel potential target for the design of contraception by blocking sperm movement or capacitation.

精子获能依赖于cAMP/PKA信号通路，而获能时超活化运动的启动又依赖于Ca2+信号通路，然而这两种信号通路之间相互关系尚不明确。申请者率先发现一种纤维鞘FSCB/CABYR蛋白复合物，该复合物既参与了cAMP/PKA信号的转导，也具有钙结合能力，参与钙信号转导。为此，本课题拟通过利用本研究组前期FSCB单基因敲除小鼠，进一步联合敲除CABYR基因，探讨该复合物功能缺失对精子运动和获能的影响。本研究对于理解获能的分子机制具有重要理论意义，且可能为开发经阻断精子运动和获能进行避孕的药物提供新靶点。