格特隐球菌逃逸巨噬细胞非特异性免疫应答的机制研究

Cryptococcus gattii is an emerging fungal pathogen that causes a potentially fatal infection in healthy individuals. It is important to study the pathogenic mechanism because of its increasing infections in China. We analyzed the genotype characteristics of 82 strains of Cryptococcus neoformans/ Cryptococcus gattii in China from clinical samples. Finally we successfully gained 3 strains of Cryptococcus gattii. Our previous studies showed that the capsule was a characteristic physiological structure of Cryptococcus gattii and mediated the immune escape of macrophage phagocytosis. The capsule played an important biological role in Cryptococcus gattii infections. This research aims to screen and analyze the genotype characteristics of Cryptococcus gattii in China, explore the biological role of capsule on macrophage phagocytosis and related signaling, elucidate the potential mechanisms of Cryptococcus gattii infections, in order to provide experimental basis for effective pathogenesis of Cryptococcus gattii and clinical treatment.

格特隐球菌是我国近几年发现的一种致命性真菌，导致免疫正常宿主发生肺部感染及脑部感染。该菌在国外出现过多次爆发，我国虽然没有爆发但也在逐年增加，因此研究其致病机制至关重要。我们前期从82例隐球菌中分离出来了3例格特隐球菌菌株。初步结果显示，新生隐球菌均能被巨噬细胞吞噬，引起一系列的非特异性免疫反应；而格特隐球菌却能够逃逸巨噬细胞的吞噬，进而逃避宿主的非特异性免疫反应。随后体内、体外实验发现格特隐球菌荚膜较新生隐球菌显著增厚，可能介导了该菌对巨噬细胞吞噬效应的免疫逃逸。因此本课题的研究目的旨在揭示其逃避巨噬细胞非特异性免疫反应的可能机制，以期对格特隐球菌的致病机制和临床治疗提供有效的实验依据。

格特隐球菌是我国近几年发现的一种致命性真菌，导致免疫正常宿主发生肺部感染及脑部感染。该菌在国外出现过多次爆发，我国虽然没有爆发但也在逐年增加，因此研究其致病机制至关重要。我们前期从82例隐球菌中分离出来了3例格特隐球菌菌株。初步结果显示，新生隐球菌均能被巨噬细胞吞噬，引起一系列的非特异性免疫反应；而格特隐球菌却能够逃逸巨噬细胞的吞噬，进而逃避宿主的非特异性免疫反应。随后体内、体外实验发现格特隐球菌荚膜较新生隐球菌显著增厚，可能介导了该菌对巨噬细胞吞噬效应的免疫逃逸。因此本课题的研究目的旨在揭示其逃避巨噬细胞非特异性免疫反应的可能机制，以期对格特隐球菌的致病机制和临床治疗提供有效的实验依据。